Aquaculture effluent treatment with ammonia remover Bacillus albus (ASSF01). Issue 4 (August 2021)

• Record Type: Journal Article
• Authors: Gogoi, Mandakini; Bhattacharya, Pinaki; Kumar Sen, Sudip; Mukherjee, Indranil; Bhushan, Shashi; Chaudhuri, Shaon Ray
• Is Part Of: Journal of environmental chemical engineering. Volume 9:Issue 4(2021)
• Publication Date: 2021-08
• Subjects: Ammonia -- Aquaculture effluent -- Nitrite -- Immobilized condition; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2021.105697
• Languages: English
• ISSNs: 2213-2929

Abstract

Abstract: Ammonia toxicity in water bodies is a global problem. Different approaches have been adopted to get the ammonia concentration in water back to permitted level (0.5 mg L −1 for aquaculture and 5 mg L −1 for environmental discharge). The existing techniques are elaborate and time consuming, often not economically viable. This study reports a single unit packed bed biofilm reactor-based treatment of Aquaculture Effluent (AE) and non-AE using a pure culture of Bacillus albus ASSF01 from environmental origin as inoculum. The strain could reduce an initial ammonia concentration of 1.7 mg L −1 to 4.7 mg L −1 to a final ammonia concentration of 0.5 mg L −1 within 12–16 h while that of 27 mg L −1, 47 mg L −1 and 100 mg L −1 to 5 mg L −1 within 42 h, 48 h, and 98 h under immobilized condition respectively. Upon scaling up the operation to 9.5 L at ambient temperature, the strain could maintain its efficiency along with simultaneous reduction of nitrite from 0.30 mg L −1 to the accepted level of 0.01 mg L −1 . The parameters optimized during batch mode operation using Response surface methodology were also applicable for continuous mode operation. The isolate under immobilized condition could remove ammonia at a concentration of 7.92 mg kg −1 of matrix from Luria Bertani broth while 41.6 mg kg −1 of matrix from minimal medium indicating it to be suitable for environmental application. This study reports a rapid, single unit bacterial biofilm based stable continuous mode treatment strategy for ammonia removal from effluent ensuring environmental protection. Graphical Abstract: ga1 Highlights: Bacillus albus ASSF01 is an efficient ammonia remover and stable biofilm former. It removes ammonia from Aquaculture Effluent from 3.2 mg L −1 to 0.5 mg L −1 in 14 h. The isolate could reduce nitrite from 0.30 mg L −1 to 0.01 mg L −1 during treatment. Continuous mode biofilm based treatment strategy ensures environmental protection.