Synergistic supplementation of organic carbon substrates for upgrading neutral lipids and fatty acids contents in microalga. Issue 4 (August 2021)

• Record Type: Journal Article
• Authors: Whangchai, Kanda; Mathimani, Thangavel; Sekar, Manigandan; Shanmugam, Sabarathinam; Brindhadevi, Kathirvel; Van Hung, Tran; Chinnathambi, Arunachalam; Alharbi, Sulaiman Ali; Pugazhendhi, Arivalagan
• Is Part Of: Journal of environmental chemical engineering. Volume 9:Issue 4(2021)
• Publication Date: 2021-08
• Subjects: Microalgae -- Mixotrophy -- Organic carbon -- Acetate -- Lipids -- Biodiesel; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2021.105482
• Languages: English
• ISSNs: 2213-2929

Abstract

Abstract: Phototrophic and mixotrophic cultivation of C. vulgaris for ameliorating biomass yield, lipid content, neutral lipid content and fatty acid composition was investigated. Phototrophic cultivation of C. vulgaris produced 1.28 g/L dry cell weight and 15.3% lipid content on day 18 whereas in mixotrophic mode of cultivation, acetate at 0.4% has yielded higher dry cell weight of 2.7 g/L and 20.8% lipids compared to dextrose and bicarbonate. Central composite design (CCD) based optimization showed combined supplementation of 0.4% acetate + 0.6% dextrose has accumulated higher lipid content of 27% and 69% neutral lipid compared to acetate + bicarbonate, and dextrose + bicarbonate conditions. Further, fatty acid composition of acetate + dextrose supplemented cells possessed high levels of C16:0, C16:1 and C18:1, which makes mixotrophically cultivated C. vulgaris for pilot-scale production. Graphical Abstract: ga1 Highlights: C. vulgaris was cultured under phototrophic and mixotrophic conditions for lipid. Dextrose, acetate and bicarbonate were used as organic, exogenous carbon sources. Acetate at 0.4% has yielded higher DCW and lipids than phototrophic condition. CCD showed that 0.4% acetate + 0.6% dextrose accumulated higher lipids about 27%. Acetate + dextrose condition showed high levels of C16:0, C16:1 and C18:1.