A novel "off-on-off" fluorescent probe for sensing of Fe3+ and F− successively in aqueous solution and its application in cells. (October 2021)

• Record Type: Journal Article
• Authors: Li, Bai; Gu, Xin; Wang, Minghui; Liu, Xuejun; Xu, Kuoxi
• Is Part Of: Dyes and pigments. Volume 194(2021)
• Publication Date: 2021-10
• Subjects: Fluorescent probe -- "Off-on-off" response -- Fe3+ -- F− -- Fluorescence imaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2021.109637
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: We have successfully developed a bis-schiff base fluorescent probe L, linking hemicyanine aldehyde and salicylaldehyde through hydrazine hydrate, which continuously identify Fe 3+ and F − in MeCN-Tris (v/v, 2:1, 10 mM, pH 7.3) solution. The probe L showed a highly selective and sensitive fluorescence "turn-on" for Fe 3+ over other metal ions accompanying with the solution color change from yellow to red. Furthermore, the resulting L-Fe 3+ complex detect only F − among the common various anions. The bonding mechanisms were detected by fluorescence and absorption titrations, 1 H NMR titrations, HRMS and theoretical calculations. In addition, the probe has been used for liquid colorimetry and test paper colorimetric identification of Fe 3+ . Moreover, the probe L was used for sequentially off-on-off fluorescence imaging of Fe 3+ and F − in HeLa cells. Graphical abstract: In this study, we have successfully developed a bis-schiff base fluorescent probe L, linking hemicyanine aldehyde and salicylaldehyde through hydrazine hydrate, which continuously identify Fe 3+ and F − in MeCN-Tris (v/v, 2:1, 10 mM, pH 7.3) solution. The probe L showed a highly selective and sensitive fluorescence "turn-on" for Fe 3+ over other metal ions accompanying with the solution color change from yellow to red. Furthermore, the resulting L-Fe 3+ complex detect only F − among the common various anions. The bonding mechanisms were detected by fluorescence and absorption titrations, 1 H NMR titrations, HRMS and theoretical calculations. In addition, the probe has been used for liquid colorimetry and test paper colorimetric identification of Fe 3+ . Moreover, the probe L was used for sequentially off-on-off fluorescence imaging of Fe 3+ and F − in HeLa cells. Image 1 Highlights: A new hemicyanidine-based colorimetric-fluorescent probe L was synthesized. The probe L can detect Fe 3+ and F − ions in aqueous solutions. The binding modes of probe L with Fe 3+ have been well demonstrated by MS, 1 H NMR and DFT calculation. The probe L had the potential application value for monitoring Fe 3+ and F − ions in environment and vivo.