A robust camphor-based colorimetric and fluormetric dual-modal probe with a large Stokes shift for real-time monitoring of endogenous labile Fe2+ in vivo and in vitro. (October 2021)

• Record Type: Journal Article
• Authors: Zhang, Yan; Gong, Shuai; Song, Jie; Li, Mingxin; Qin, Ahui; Gao, Yu; Wang, Zhonglong; Wang, Shifa
• Is Part Of: Dyes and pigments. Volume 194(2021)
• Publication Date: 2021-10
• Subjects: Camphor -- Fluorescent probe -- Labile Fe2+ -- Ferroptosis -- Bioimaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2021.109590
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Fe 2+ plays a critical role in many physiological processes in the human body. The abnormal level of Fe 2+ is implicated in a variety of diseases ranging from anemia to heart failure and even cancer. Herein, a new camphor-derived colorimetric and fluormetric dual-modal probe CCO was constructed for real-time monitoring and imaging of Fe 2+ in vitro and vivo. The probe CCO manifested excellent selectivity, superior sensitivity (LOD = 8.3 nM), large Stokes shift (~208 nm), rapid reaction time (<40 s), and remarkable "turn-on" fluorescence response (~21-fold) toward Fe 2+ in aqueous solution. With this probe in hand, it is feasible to image the labile Fe 2+ supplementation and depletion in living cells. Moreover, this probe was successfully applied to visualize labile Fe 2+ generation in living cells and zebrafish larvae under ferroptosis model, which would provide a highly effective tool for in-depth understanding its physiological and pathological functions. Highlights: A novel "turn-on" fluorescent probe CCO for Fe 2+ was synthesized from camphor. CCO exhibited colorimetric and fluormetric dual responses toward Fe 2+ . CCO was sensitive enough to monitor exogenous and endogenous Fe 2+ in Hela cells. CCO can rapidly respond to labile Fe 2+ in living cells and zebrafish under ferroptosis model.