Characteristics of a novel heterotrophic nitrification-aerobic denitrification yeast, Barnettozyma californica K1. (November 2021)

• Record Type: Journal Article
• Authors: Fang, Jinkun; Liao, Shaoan; Zhang, Shanshan; Li, Li; Tan, Simin; Li, Wenzhuo; Wang, Anli; Ye, Jianmin
• Is Part Of: Bioresource technology. Volume 339(2021)
• Publication Date: 2021-11
• Subjects: Heterotrophic nitrification -- Aerobic denitrification -- Barnettozyma californica -- Inorganic nitrogen removal; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125665
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Barnettozyma californica K1 has heterotrophic nitrification-aerobic denitrification ability. High removal efficiencies of inorganic nitrogen were found. Strain K1 is able to utilize different carbon sources in HN-AD. Strain K1 harbors the functional genes amoA, nirK and nosZ . Abstract: Yeast strain K1, isolated from surface sediment, was identified as Barnettozyma californica . The strain showed efficient heterotrophic nitrification and aerobic denitrification (HN-AD) at initial ammonium, nitrite and nitrate concentrations of 14–140 mg/L. Additionally, the optimum carbon source for its growth and nitrogen removal activity was sucrose, followed by glucose, acetate and citrate. The maximum removal efficiencies of ammonium, nitrite and nitrate were 99.11%, 99.13% and 98.84% under 48 h of culture with sucrose at 140 mg/L nitrogen and the corresponding removal efficiencies of total nitrogen were 90.16%, 86.65% and 81.48%, respectively. The optimum conditions for the inorganic nitrogen removal and growth of strain K1 were a C/N of 18 and a salinity of 5-15 ppt. The amoA, nirK and nosZ genes of K1 were detected. All the results suggest that B. californica K1 is capable of HN-AD and has the potential to remove inorganic nitrogen from wastewater.