Lethal dose and histopathological alterations induced by Aeromonas salmonicida in experimentally challenged common carp, Cyprinus carpio. (September 2021)

• Record Type: Journal Article
• Authors: Hussain Bhat, Raja Aadil; Thakuria, Dimpal; Dubey, Maneesh Kumar; Tandel, Ritesh Shantilal; Sharma, Prakash; Khangembam, Victoria C.; Dash, Pragyan; Tripathi, Gayatri; Sarma, Debajit
• Is Part Of: Microbial pathogenesis. Volume 158(2021)
• Publication Date: 2021-09
• Subjects: Aeromonas salmonicida -- Common carp -- Histopathology -- LD50; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2021.105110
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Aeromonas salmonicida is the obligate pathogen of fishes having zoonotic potential. It is reported to cause considerable losses in world aquaculture. The current study has successfully demonstrated the induction of histopathological lesions in experimentally infected common carp. In the current study, the lethal concentration (LD50 -96 h) of typical A. Salmonicida for common carp was found to be 1.5 × 10 7 CFU mL −1 . About 40% and 60% fish mortalities occurred after 72 h in the groups inoculated with 10 7 and 10 8 CFU mL −1 bacterial suspension, respectively. The fish challenged with A. salmonicida showed symptoms like abnormal swimming behaviour, lethargy, intra-abdominal fluid, haemorrhages on the ventral side of the body, vent and fins. The signs proceeded with the death of fish. In the histological sections, severe pathological alterations were reported in the tissue sections of internal organs. The microscopic observation showed sinusoidal and large blood vessel congestion in the liver, profuse haemorrhage, necrosis and infiltration of blood cells in the internal organs. The tubular architecture was lost with the infiltration of leucocytes in the kidney. In gills, more intense and prominent lamellar fusion was observed with leucocytic infiltration, telangiectasia and hyperplasia of lamellar epithelial cells. In summary, we have experimentally induced the typical A. salmonicida infection in common carp. The study will provide a research foundation for further studies on the host-pathogen interaction, therapeutics and epidemiology of A. salmonicida . Highlights: LD50 -96 h of Aeromonas salmonicida for common carp ( Cyprinus carpio ) was 1.5 × 10 7 CFU mL −1 . Inoculation of 10 7 and 10 8 CFU mL −1 bacterial suspension caused 40% and 60% fish mortalities respectively after 72 h. Abnormal swimming behaviour, lethargy, intra-abdominal fluid, haemorrhages on body surface, vent and fins were observed. In the histological sections, severe pathological alterations were observed in internal organs.