Mitochondria-targeted near-infrared fluorescent probe for monitoring of a Copper(I) in living cells and In vivo. (October 2021)

• Record Type: Journal Article
• Authors: Qian, Xiaoli; Zhu, Wenchao; Yu, Hui; Xu, Yue; Liu, Wangwang; Wang, Hai-Yan; Liu, Yi
• Is Part Of: Dyes and pigments. Volume 194(2021)
• Publication Date: 2021-10
• Subjects: NIR -- Fluorescent probes -- Copper -- In vivo -- Efficacy evaluation; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2021.109561
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Non-invasive and accurate detection of copper is essential to elucidate its functions. Herein, we report a mitochondria-targeted near-infrared probe (NFL-TPA) for bioimaging of copper. The probe exhibits high sensitivity and selectivity for copper(I) with low detect of limit (14.4 nM). More importantly, the probe can monitor the level of copper(I) in mitochondria, and successfully detect the distribution of copper(I) in living mice by near infrared fluorescence (NIR) imaging in vivo and ex vivo . Furthermore, the probe could assess the therapeutic efficiency of d -penicillamine. Thus, the probe motivates the development of novel NIR platform for copper(I) detection and drug evaluation. Highlights: A mitochondria-targeted NIR sensor (NFL-TPA) was developed for detecting copper(I). The probe exhibits excellent sensitivity and high selectivity for copper(I) with low limit of detection (14.4 nM). The probe could monitor the level of copper(I) in mitochondria, and sensitively response to copper(I) in living cells and mice. A NIR sensor (NFL-TPA) could sensitively response to copper(Ⅰ) and evaluate the therapeutic efficacy of D-penicillamine.