Actinomyces qiguomingii sp. nov., isolated from the Pantholops hodgsonii. Issue 1 (14th October 2019)

• Record Type: Journal Article
• Authors: Zhu, Wentao; Yang, Jing; Lu, Shan; Lai, Xin-He; Jin, Dong; Wang, Xiaoxia; Pu, Ji; Ren, Zhihong; Huang, Ying; Wu, Xiaomin; Zhang, Xiaoyan; Xu, Jianqing; Xu, Jianguo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 58
• Page End: 64
• Publication Date: 2019-10-14
• Subjects: Actinomyces qiguomingii sp. nov. -- Tibetan antelope -- Qinghai-Tibet Plateau; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003709
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410 T and 553, were isolated from faeces of the Tibetan antelope ( Pantholops hodgsonii ) from the Tibet-Qinghai Plateau, PR China. The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7. The end products from glucose fermentation included ethanol and lactic acid. Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410 T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %). The genomic G+C content of strain 410 T was 67.4 mol%. Digital DNA–DNA hybridization values between strain 410 T and each of the closely related species were under 70 %. The respiratory quinones were MK-10 (68 %) and MK-9 (32 %). The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω 9 c . The major polar lipids were diphosphatidylglycerol and phosphatidylinositol-mannoside. The whole-cell sugars contained rhamnose, ribose and glucose. The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine. The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyce s qiguomingii sp. nov. is proposed. The type strain is 410 T (=CGMCC 1.16361 T = DSM 106201 T ).