Flavobacterium cellulosilyticum sp. nov., a novel psychrophilic bacterium isolated from Arctic soil. Issue 1 (11th September 2019)

• Record Type: Journal Article
• Authors: Chaudhary, Dhiraj Kumar; Khulan, Altankhuu; Kim, Dong-Uk; Kim, Jaisoo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 1(2020)
• Page Start: 44
• Page End: 50
• Publication Date: 2019-09-11
• Subjects: Flavobacterium cellulosilyticum sp. nov. -- Arctic soil -- Flavobacteriaceae -- psychrophilic -- Bacteroidetes; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003707
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel psychrophilic, light-yellow-coloured, aerobic, Gram-stain-negative, rod-shaped, non-motile bacterium, designated strain AR-3-4 T was isolated from a sample of Arctic soil. Strain AR-3-4 T grew at 0–25 °C, pH 6.0–9.0 and 0–1.0 % (w/v) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain AR-3-4 T belonged to the genus Flavobacterium, with nearest phylogenetic neighbour being Flavobacterium fluvii H7 T (97.5 % sequence similarity). The strain comprised phosphatidylethanolamine as the main polar lipid, MK-6 as predominant respiratory quinone, and summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6 c ), anteiso-C15 : 0 and iso-C15 : 0 as the major fatty acids. The average nucleotide identity and in silico DNA–DNA hybridization values between strain AR-3-4 T and closest members were below the threshold values of 95 % and 70 %, respectively. The DNA G+C content was 33.0 mol%. Based on the polyphasic taxonomic data, the novel species Flavobacterium cellulosilyticum sp. nov. is proposed with the type strain AR-3-4 T (=KEMB 9005–740 T =KACC 21171 T =NBRC 113787 T ).