A nait‐associated and previously unreported mutation in the ITGB3 gene with a low frequency in the local population. Issue 4 (16th March 2021)

• Record Type: Journal Article
• Authors: Norrenbrock, Stefan; Müller, Thomas H.; Mayer, Christiane; Doescher, Andrea
• Is Part Of: Transfusion medicine. Volume 31:Issue 4(2021)
• Page Start: 286
• Page End: 291
• Publication Date: 2021-03-16
• Subjects: human platelet antigens -- ITGB3 -- neonatal alloimmune thrombocytopenia; Blood -- Transfusion -- Periodicals; 615.39
• DOI: 10.1111/tme.12769
• Languages: English
• ISSNs: 0958-7578

Abstract

Abstract: Background: Neonatal alloimmune thrombocytopenia is a rare but potentially severe postnatal complication caused by maternal allo‐antibodies against platelet antigens of the newborn. In relatively few cases, immunisation against low‐frequency antigens has been reported. Methods: Platelet antigens of a newborn with severe thrombocytopenia and his family members were investigated by serological and molecular biological methods. A real‐time PCR assay was developed to reliably detect this mutation in pools of DNA from up to seven individuals. Results: Serological testing showed positive reactions of maternal plasma with paternal platelets but not with conventional platelet donor panels. Sequencing of the ITGB3 gene revealed a G > A polymorphism in position c.1915 of exon 12 for the father, the newborn and three of four paternal relatives. Screening of samples from a local population of 1575 Caucasian blood donors identified only a single individual with this mutation. Conclusion: This finding of a previously unreported private platelet antigen demonstrates that the identification of the target glycoprotein by MAIPA assay followed by sequencing of the affected gene can be combined with an efficient population screening by real‐time PCR with pooling of DNA samples.