P759 Evaluation of the fully automated bio-rad bioplex 2200 syphilis total & RPR antibody detection assay. (14th July 2019)

• Record Type: Journal Article
• Authors: Boissonneault, Vincent; Martel-Laferrière, Valérie; Serhir, Bouchra; Fortin, Claude
• Is Part Of: Sexually transmitted infections. Volume 95(2019)Supplement 1
• Page Start: A326
• Page End: A326
• Publication Date: 2019-07-14
• Subjects: syphilis; Sexually transmitted diseases -- Periodicals; HIV infections -- Periodicals; 616.951005
• DOI: 10.1136/sextrans-2019-sti.817
• Languages: English
• ISSNs: 1368-4973

Abstract

Abstract : Background: Laboratory diagnostic of syphilis is based on both treponemal and non-treponemal assays. While automated treponemal assays are available, non-treponemal antibody testing continue to require extensive hands on time. The BioPlex 2200 Syphilis Total & RPR assay by Bio-Rad Laboratories is a fully automated assay that detects both treponemal and non-treponemal antibodies with on board RPR titer capability. Methods: We compared the BioPlex Total & RPR assay with the BiopPex IgG assay and with the Wampole Impact RPR card test for the treponemal and non-treponemal antibodies detection respectively. A total of 504 prospective sera sent to our laboratory for syphilis testing were tested with both BioPlex assays to establish agreement of treponemal results. Also, a panel of 52 sera with known titer values was provided by our reference laboratory. These sera were tested with the BioPlex Total & RPR assay and the manual Wampole RPR card to compare titer results, using essential agreement within 1 dilution range and correlation of log2 transformed titer values with Spearman coefficient. Results: Of the 504 sera tested, 112 were positive for treponemal antibodies with BioPlex Total & RPR and 109 with BioPlex IgG. Positive, negative and overall agreements were 95% (104/109)(95% CI [89%, 98%]), 98% (387/395)(95% CI [96%, 99%]) and 97% (491/504)(95% CI [95%, 99%]) respectively for these sera. Essential agreement within 1 dilution of quantitative RPR titer results for the 52 sera panel was 90% and the Spearman correlation coefficient of titers obtained with the two assays was 0.926 with a p <0.001. Conclusion: The BioPlex 2200 Syphilis Total & RPR assay is a fully automated assay that compares favorably with the BioPlex 2200 Syphilis IgG assay and that yields non treponemal titers that correlates favorably with a traditional manual RPR card test. Reliable automated non treponemal assays are needed. Disclosure: No significant relationships.