306 A Novel Anti-Oxidant to Counteract Oxidative Stress During Resuscitation after Birth Asphyxia. (October 2012)

• Record Type: Journal Article
• Authors: Henckel, E; Solberg, R; Calisch, TE; Norgren, S; Bohlin, K; Saugstad, OD
• Is Part Of: Archives of disease in childhood. Volume 97(2012)Supplement 2
• Page Start: A90
• Page End: A90
• Publication Date: 2012-10
• Subjects: Children -- Diseases -- Periodicals; Infants -- Diseases -- Periodicals; 618.920005
• DOI: 10.1136/archdischild-2012-302724.0306
• Languages: English
• ISSNs: 0003-9888

Abstract

Abstract : Background and Aims: Oxidative stress contributes to tissue damage after perinatal asphyxia. The thiol-containing free radical scavenger N -acetylcysteine amide (NACA) is a promising new anti-oxidant with good penetration into the mitochondria. The objective was to investigate the protective effect of NACA in a piglet model of birth asphyxia. Methods: Anesthetized newborn piglets (n=51) were subjected to global hypoxemia and block-randomized to either intravenous administration of NACA 300 mg/kg and resuscitated with 21% oxygen for 30 min, saline and 21% oxygen, NACA and 100% oxygen or saline and 100% oxygen. After resuscitation, the piglets were followed for 9 hours and samples for several markers of injury and oxidative stress were collected. Reported here are clinical parameters and measurements of reduced to oxidized gluthatione (GSH/GSSG). Results: Thirty minutes after end-resuscitation metabolic acidosis was less pronounced in the 100%-NACA group compared to 100%-oxygen-alone (lactate 8.1±2.6 vs 10.9±3.4, p<0.05). This difference was not shown for the 21%-oxygen groups. Mean arterial blood pressure and hemoglobin levels remained similar between the groups. The GSSG values were generally low. At end-resuscitation GSH was lower in 100%-NACA compared to 100%-oxygen-alone group (164±111 vs 255±113 µmol, p<0.05) and delta-GSH during resuscitation greater (143±49 vs 32±66 µmol p<0.001). Conclusions: The data indicate that NACA may enhance immediate recovery, improve mithochondrial glutatione metabolism and restore the cell to a normal metabolism following asphyxia and rescucitation. Upcoming analyses of histopathology and injury markers will further elucidate neuroprotective effect of NACA treatment following birth asphyxia.