Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry. (28th November 2012)

• Record Type: Journal Article
• Authors: Taghdiri, Mehdi; Mohamadipour-taziyan, Arash
• Other Names: Lee J. Academic Editor.; Pezza L. Academic Editor.; Zacharis C. Academic Editor.
• Is Part Of: ISRN pharmaceutics. Volume 2012(2012)
• Publication Date: 2012-11-28
• Subjects: Pharmacy -- Periodicals; Pharmacological Phenomena; Pharmacology; Pharmacy; Electronic journals; Periodical; Periodicals; 615
• DOI: 10.5402/2012/216068
• Languages: English
• ISSNs: 2090-6145

Abstract

Abstract : A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps. Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution. This attenuation was used to the microdetermination of dopamine by measurement of absorbance of the solid phase (Sephadex LH-20 gel) in a 1.0 mm cell at 625 nm. Dopamine could be determined in the concentration range of 0.4–1.6 μ g mL −1 (10-mL Sample volume) with a relative standard deviation (RSD) of 0.03% (n = 4 ). The detection limit was obtained, 0.26 μ g mL −1 (1.7 μ M). The method was used for determination of dopamine in pharmaceutical injection sample and satisfactory result was obtained.