Co-localization of the PDGF β-Receptor and Actin during PDGF Stimulation in Mouse Fibroblasts. (19th March 2012)

• Record Type: Journal Article
• Authors: Moes, Maarten J. A.; Zhou, Yeping; Boonstra, Johannes
• Other Names: Castoria G. Academic Editor.; Song W. K. Academic Editor.; Storz P. Academic Editor.
• Is Part Of: ISRN cell biology. Volume 2012(2012)
• Publication Date: 2012-03-19
• Subjects: Cytology -- Periodicals; Cells; Cell Biology; Cells; Cytological Techniques; Biology; Cells; Cytology; Periodicals; Electronic journals; 571.6
• DOI: 10.5402/2012/568104
• Languages: English
• ISSNs: 2090-7370

Abstract

Abstract : The subcellular localization of the PDGF β -receptor was investigated in relation with PDGF-induced actin and membrane dynamics in mouse C3H10T1/2 fibroblasts. Serum-starved cells exhibit a nonhomogenous distribution of PDGF β -receptors. However, the observed pattern does not resemble the localization of PDGF-induced actin structures. Interestingly, the PDGF β -receptor showed a changed subcellular distribution in relation to the formation of PDGF-BB-induced actin structures. Upon PDGF exposure, PDGF β -receptors were found to accumulate in dorsal circular ruffles. The presence of both macropinosomes and clathrin in the induced circular ruffles suggests that the accumulation of PDGF β -receptors in circular ruffles results in the efficient internalization of PDGF β -receptors.