A Novel Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Cytochrome C and Forecast of Human Diseases. (26th December 2012)

• Record Type: Journal Article
• Authors: Zheng, Zhi-Yong; Cui, Ma-Lin; Liu, Jia-ming; Jiang, Shu-Lian; Jiao, Li; Zhang, Li-Hong; Lin, Xuan; Lin, Shao-Qin
• Other Names: Alvarez-Lorenzo C. Academic Editor.; Konarev D. V. Academic Editor.; Türker A. R. Academic Editor.
• Is Part Of: ISRN spectroscopy. Volume 2012(2012)
• Publication Date: 2012-12-26
• Subjects: Spectrum analysis -- Periodicals; Spectrum analysis; Periodicals; Electronic journals; 543.5
• DOI: 10.5402/2012/918093
• Languages: English
• ISSNs: 2090-8776

Abstract

Abstract : The reaction between fullerenol (F-ol) and cytochrome C (Cyt C) could be carried out to form a nonphosphorescence compound using tween-80 as photosensitizer which causes the sharp quenching of room temperature phosphorescence (RTP) of F-ol. Bearing this in mind, a novel solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace Cyt C has been proposed in this study. Under the optimum conditions, the linear range of this method is 1.0 × 10 - 16 –2.4 × 10 - 14 g mL - 1, which is directly proportional to Δ I p of Cyt C-F-ol–tween-80 system, and the detection limit (DL) is 3.0 × 10 − 17 g mL - 1 . It has been applied to the determination of Cyt C in human serum and forecast of diseases, and the result matches with the enzyme-linked fluorescence immunoassay (ELISA). Meanwhile, the reaction mechanism of SSRTP for the determination of Cyt C and the enhancing effect of tween-80 on RTP of F-ol were also discussed.