Genetic Determinants of Methicillin Resistance and Virulence among Staphylococcus aureus Isolates Recovered from Clinical and Surveillance Cultures in a Brazilian Teaching Hospital. (17th May 2012)

• Record Type: Journal Article
• Authors: Rodrigues, Marcus Vinicius Pimenta; Branco Fortaleza, Carlos Magno Castelo; Martins Souza, Camila Sena; Teixeira, Natalia Bibiana; Ribeiro de Souza da Cunha, Maria de Lourdes
• Other Names: Blaiotta G. Academic Editor.; Netrusov A. Academic Editor.
• Is Part Of: ISRN microbiology. Volume 2012(2012)
• Publication Date: 2012-05-17
• Subjects: Microbiology -- Periodicals; Microbiology; Microbiological Phenomena; Fulltext; Internet Resources; Periodicals; 579
• DOI: 10.5402/2012/975143
• Languages: English
• ISSNs: 2090-7478

Abstract

Abstract : Aims . To quantify the presence of SCC mec types and virulence genes among Staphylococcus aureus colonizing and infecting patients from a teaching hospital. Methods . We analyzed 225 and 84 S. aureus isolates recovered from surveillance and clinical cultures, respectively. Strains were studied for the presence and type of SCC mec, as well as for several virulence genes. Univariate and multivariable analysis were performed in order to identify predictors of invasiveness (defined as isolation from clinical cultures). Results . The presence of SCC mec types III (OR, 2.19, 95% CI, 1.08–4.45) and IV (OR, 5.28 95% CI, 1.35–20.63) and of genes coding for exfoliative toxin B ( etb, OR, 6.38, 95% CI, 1.48–27.46) and Panton-Valentine leukocidin ( pvl, OR, 2.38, 95% CI, 1.16–4.86) was independently associated with invasiveness. Conclusions . SCC mec types III and IV and virulence genes are associated with greater invasiveness of S. aureus . Patients colonized with methicillin-resistant S. aureus, as well as with strains harboring etb or pvl, may be prone to develop invasive disease. Infection-preventing strategies should be more intensively applied to this group.