Unbiased interrogation of memory B cells from convalescent COVID-19 patients reveals a broad antiviral humoral response targeting SARS-CoV-2 antigens beyond the spike protein. (August 2021)

• Record Type: Journal Article
• Authors: DiMuzio, Jillian M.; Heimbach, Baron C.; Howanski, Raymond J.; Dowling, John P.; Patel, Nirja B.; Henriquez, Noeleya; Nicolescu, Chris; Nath, Mitchell; Polley, Antonio; Bingaman, Jamie L.; Smith, Todd; Harman, Benjamin C.; Robinson, Matthew K.; Morin, Michael J.; Nikitin, Pavel A.
• Is Part Of: Vaccine. Volume 8(2020)
• Publication Date: 2021-08
• Subjects: Convalescent plasma -- COVID-19 -- Antibody response -- B cell repertoire
• DOI: 10.1016/j.jvacx.2021.100098
• Languages: English
• ISSNs: 2590-1362

Abstract

Abstract: Patients who recover from SARS-CoV-2 infections produce antibodies and antigen-specific T cells against multiple viral proteins. Here, an unbiased interrogation of the anti-viral memory B cell repertoire of convalescent patients has been performed by generating large, stable hybridoma libraries and screening thousands of monoclonal antibodies to identify specific, high-affinity immunoglobulins (Igs) directed at distinct viral components. As expected, a significant number of antibodies were directed at the Spike (S) protein, a majority of which recognized the full-length protein. These full-length Spike specific antibodies included a group of somatically hypermutated IgMs. Further, all but one of the six COVID-19 convalescent patients produced class-switched antibodies to a soluble form of the receptor-binding domain (RBD) of S protein. Functional properties of anti-Spike antibodies were confirmed in a pseudovirus neutralization assay. Importantly, more than half of all of the antibodies generated were directed at non-S viral proteins, including structural nucleocapsid (N) and membrane (M) proteins, as well as auxiliary open reading frame-encoded (ORF) proteins. The antibodies were generally characterized as having variable levels of somatic hypermutations (SHM) in all Ig classes and sub-types, and a diversity of VL and VH gene usage. These findings demonstrated that an unbiased, function-based approach towards interrogating the COVID-19 patient memory B cell response may have distinct advantages relative to genomics-based approaches when identifying highly effective anti-viral antibodies directed at SARS-CoV-2.