Co-delivery of quercetin and caffeic-acid phenethyl ester by polymeric nanoparticles for improved antitumor efficacy in colon cancer cells. (18th August 2021)

• Record Type: Journal Article
• Authors: Colpan, Reyhan Dilsu; Erdemir, Aysegul
• Is Part Of: Journal of microencapsulation. Volume 38:Number 6(2021)
• Page Start: 381
• Page End: 393
• Publication Date: 2021-08-18
• Subjects: Quercetin -- caffeic-acid phenethyl ester -- nanoparticle -- colon cancer -- anti-cancer; Microencapsulation -- Periodicals; Controlled release technology -- Periodicals; 615.19
• DOI: 10.1080/02652048.2021.1948623
• Languages: English
• ISSNs: 0265-2048

Abstract

Abstract: Aim: This study aimed to synthesise quercetin- caffeic-acid phenethyl ester (CAPE)-co-loaded poly(lactic-co-glycolic-acid) (PLGA) nanoparticles (QuCaNP) and investigate their anti-cancer activity on human colorectal carcinoma HT-29 cells. Methods: QuCaNPs were synthesised using single-emulsion (o/w) solvent evaporation method. Particle size, zeta potential, polydispersity index, in vitro release profile, and surface morphology of QuCaNPs were determined. Cytotoxicity, anti-migration, anti-proliferation and apoptotic activities of QuCaNPs were studied. Results: Mean diameter of QuCaNP was 237.8 ± 9.670 nm, with a polydispersity index (PDI) of 0.340 ± 0.027. Encapsulation efficiency was 74.28% (quercetin) and 65.24% (CAPE). Particle size and drug content of QuCaNP remained stable for 30 days at −20 °C. The half-maximal inhibitory concentration (IC50 ) values of QuCaNP-treated HT-29 cells were calculated as 11.2 µg/mL (24 h) and 8.2 µg/mL (48 h). QuCaNP treatment increased mRNA levels of caspase-3 (2.38 fold) and caspase-9 (2-fold) and expressions of key proteins in the intrinsic apoptosis pathway in HT-29 cells. Conclusion: Overall, our results demonstrated QuCaNPs exhibits improved anti-cancer activity on HT-29 cells.