Intensity of antigen expression reflects IGHV mutational status and Dohner-defined prognostic categories in chronic lymphocytic leukemia, monoclonal B-cell lymphocytosis, and small lymphocytic lymphoma. Issue 8 (3rd July 2021)

• Record Type: Journal Article
• Authors: Balakrishna, Jayalakshmi; Basumallik, Neil; Matulonis, Robert; Scott, Drake; Salem, Dalia; Jasper, Gregory; Wiestner, Adrian; Stetler-Stevenson, Maryalice; Marti, Gerald; Sun, Clare; Yuan, Constance M.
• Is Part Of: Leukemia & lymphoma. Volume 62:Issue 8(2021)
• Page Start: 1828
• Page End: 1839
• Publication Date: 2021-07-03
• Subjects: Flow cytometry -- chronic lymphocytic leukemia -- antibody binding capacity -- antigen quantitation -- mean fluorescence intensity -- prognosis; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.1080/10428194.2021.1894641
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract: We demonstrate the prognostic utility of antigen quantitation in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and monoclonal B-cell lymphocytosis (MBL). Median antibody-bound-per-cell (ABC) of CD20, CD22, CD25, CD19, and %CD38(+) was determined in CLL (185/208), SLL (8/208) and MBL (15/208) cases by flow cytometry, then compared to Dohner-classification, immunoglobulin status (mutated, IGHV-M; unmutated, IGHV-U), CLL-IPI risk and time to first treatment (TTFT). Trisomy 12 cases showed increased %CD38-expression ( p = .0379). Higher %CD38 was observed in IGHV-U versus IGHV-M ( p = .0003). CD20ABC was increased in IGHV-U versus IGHV-M ( p = .006). Del13q cases demonstrated lower CD22ABC ( p = .0198). Cases without cytogenetic abnormality exhibited higher CD19ABC ( p = .0295) and CD22ABC ( p = .0078). Del17p cases demonstrated lower CD25ABC ( p = .0097). High and very-high CLL-IPI risk groups were associated with high CD38-expression ( p = .02) and low CD25ABC ( p = .0004). Shortened TTFT was associated with high CD38-expression ( p < .0001). Interestingly, high CD25ABC trended toward shortened TTFT ( p = .07). Quantitative antigen expression reflects CLL-IPI risk groups and Dohner-classification.