Comparisons of Vitreal Angiogenic, Inflammatory, Profibrotic Cytokines, and Chemokines Profile between Patients with Epiretinal Membrane and Macular Hole. (14th July 2021)

• Record Type: Journal Article
• Authors: Chen, Lu; Zhang, Weiwei; Xie, Ping; Ji, Jiangdong; Qian, Huiming; Yuan, Songtao; Liu, Qinghuai; Hu, Zizhong
• Other Names: Liang Hong Academic Editor.
• Is Part Of: Journal of ophthalmology. Volume 2021(2021)
• Publication Date: 2021-07-14
• Subjects: Ophthalmology -- Periodicals; Eye Diseases; Ophthalmology; Ophthalmology; Electronic journals; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 617.7
• DOI: 10.1155/2021/9947250
• Languages: English
• ISSNs: 2090-004X

Abstract

Abstract : Objectives . Idiopathic epiretinal membrane (iERM) or idiopathic macular hole (iMH) is frequently used as a "healthy" control in comparison of vitreous cytokines with other vitreoretinal diseases. This study aimed to investigate if there is a difference in vitreal cytokines expression between patients with iERM and iMH. Methods . In this prospective study, all subjects received standard pars plana vitrectomy surgery, and 0.5 ml of native vitreous sample was extracted during the vitrectomy. Luminex technology and enzyme-linked immunosorbent assay were used to profile the concentration of 52 classic angiogenic, inflammatory, and profibrotic cytokines and chemokines. Statistical analyses were performed by the Mann–Whitney U test, followed by multiple comparisons by the Bonferroni correction. Results . Vitreal samples from 13 iERM and 24 iMH were studied. Of the 52 tested cytokines, 41 were similar in expression, and 5 were under the detection limit, while 6 cytokines (MMP-8, Eotaxin, MIP-1a, RANTES, TGF- β 2, and IL-4) were differently expressed between two groups (p < 0.05 ). Nevertheless, these significances disappeared after the adjustment of Bonferroni correction. Conclusion . The tested cytokines showed similar expression between iERM and iMH patients. This indicates that eyes with iERM or iMH can be together served as "healthy" controls.