An integrated microfluidic detection system for the automated and rapid diagnosis of high-risk human papillomavirus. Issue 16 (15th July 2021)

• Record Type: Journal Article
• Authors: Zhao, Xiaoyu; Li, Xiang; Yang, Weihao; Peng, Jiwei; Huang, Jiajun; Mi, Shengli
• Is Part Of: Analyst. Volume 146:Issue 16(2021)
• Page Start: 5102
• Page End: 5114
• Publication Date: 2021-07-15
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an00623a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : The centrifugal microfluidic detection system employs loop–mediated isothermal amplification (LAMP) assay and provides sample-in-answer-out nucleic acid detection of Human papillomavirus (HPV). Abstract : Human papillomavirus (HPV) causes the prevalent sexually transmitted infection that accounts for the majority of cervical cancer incidences. Therefore, the development of a rapid, accurate, automatic and affordable nucleic acid detection strategy is urgently required for HPV tests, among which microfluidic chip is a promising diagnostic method. In this work, we developed a microfluidic detection system consisting of a microfluidic chip and the corresponding detection equipment to diagnose high-risk HPV. The proposed method integrates nucleic acid purification, isothermal amplification and real-time fluorescence detection into one device. Moreover, it demonstrates good detection performance such as high specificity of primer sets (100%) and exceptional stability (coefficient of variation <6%) among five HPV genotypes. Besides, the microfluidic loop–mediated isothermal amplification (LAMP) assay is accurate (specificity of 91.7% and sensitivity of 100%) and fast (average time threshold = 10.56 minutes) when considering the conventional qPCR assay as the gold standard. The integrated microfluidic detection system offers automated and rapid diagnosis within 40 minutes and shows broad potential to deliver point-of-care detection in resource-limited circumstances owing to its simplicity and affordability.