3D printing of Cu-doped bioactive glass composite scaffolds promotes bone regeneration through activating the HIF-1α and TNF-α pathway of hUVECs. (8th July 2021)

• Record Type: Journal Article
• Authors: Dai, Qiyuan; Li, Qingtao; Gao, Huichang; Yao, Longtao; Lin, Zefeng; Li, Dingguo; Zhu, Shuangli; Liu, Cong; Yang, Zhen; Wang, Gang; Chen, Dafu; Chen, Xiaofeng; Cao, Xiaodong
• Is Part Of: Biomaterials science. Volume 9:Number 16(2021)
• Page Start: 5519
• Page End: 5532
• Publication Date: 2021-07-08
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d1bm00870f
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : A 3D-printed Cu-BG composite scaffold promoted subsequent angiogenesis and osteogenesis coupling behavior through the HIF-1α and TNF-α pathway of hUVECs. Abstract : The increasing insight into the molecular and cellular processes within the angiogenic cascade assists in enhancing the survival and integration of engineered bone constructs. Copper-doped bioactive glass (Cu-BG) is now a potential structural component of the novel scaffolds and implants used in orthopedic and dental repairs. However, it is difficult for BG, especially micro-nano particles, to be printed into scaffolds and still retain its biological activity and ability to biodegrade. Additionally, the mechanisms of the copper-stimulating autocrine and paracrine effects of human umbilical vein endothelial cells (hUVECs) during repair and regeneration of bone are not yet clear. Therefore, in this study, we created monodispersed micro-nano spherical Cu-BG particles with varying copper content through a sol–gel process. Through in vitro tests, we found that Cu-BG enhanced angiogenesis by activating the pro-inflammatory environment and the HIF-1α pathway of hUVECs. Furthermore, 2Cu-BG diluted extracts directly promoted the osteogenic differentiation of mouse bone mesenchymal stem cells (BMSCs) in vitro . Then, a new 3D-printed tyramine-modified gelatin/silk fibroin/copper-doped bioactive glass (Gel/SF/Cu-BG) scaffold for rat bone defects was constructed, and the mechanism of the profound angiogenesis effect regulated by copper was explored in vivo . Finally, we found that hydrogel containing 1 wt% 2Cu-BG effectively regulated the spatiotemporal coupling of vascularization and osteogenesis. Therefore, Cu-BG-containing scaffolds have great potential for a wide range of bone defect repairs.