Shewanella cyperi sp. nov., a facultative anaerobic bacterium isolated from mangrove sediment. Issue 8 (4th August 2021)

• Record Type: Journal Article
• Authors: Zhang, Qi; Liu, Guohong; Narsing Rao, Manik Prabhu; Tang, Rong; Yang, Shang; Ye, Wen-Yuan; Zhou, Shun-Gui; Li, Wen-Jun
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 8(2021)
• Publication Date: 2021-08-04
• Subjects: Cyperus malaccensis -- facultative anaerobic -- rhizosphere -- Shewanella cyperi sp. nov; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004940
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two Gram-stain-negative, motile, facultatively anaerobic, rod-shaped strains, FJAT-53720 T and FJAT-53726, were isolated from rhizosphere sediment of plant Cyperus malaccensis . Phylogenetic analysis based on their 16S rRNA gene sequences revealed that strains FJAT-53720 T and FJAT-53726 were affiliated to the genus Shewanella (forming an independent cluster) with the highest sequence similarity to the type strain of Shewanella algae . Optimum growth of both strains was observed at 30 °C and pH 7. The respiratory quinones were Q-7, Q-8 and MK-7. The polar lipid profile included phosphatidylmethyl ethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified aminophospholipid and four unknown phospholipids. The major fatty acids of strains FJAT-53720 T and FJAT-53726 were iso-C15:0, C17 : 1 ω 8 c and summed feature 3. The genomic DNA G+C content of strain FJAT-53720 T was 55.6 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between FJAT-53720 T and FJAT-53726 were 97.4 and 77.9 %, confirming that they were similar species and hence FJAT-53720 T was selected for further analysis. The ANI and dDDH values between FJAT-53720 T and other members of the genus Shewanella were below the cut-off level (95–96 %; 70 %) for species delineation. Based on the above results, FJAT-53720 T represents a novel species of the genus Shewanella, for which the name Shewanella cyperi sp. nov. is proposed. The type strain is FJAT-53720 T (=KCTC 82444 T =GDMCC 1.2207 T ).