Nanoporous iron oxide nanoparticle: hydrothermal fabrication, human serum albumin interaction and potential antibacterial effects. Issue 7 (9th April 2021)

• Record Type: Journal Article
• Authors: Mehrabi, Masoumeh; Ghasemi, Mohammad Faezi; Rasti, Behnam; Falahati, Mojtaba; Mirzaie, Amir; Hasan, Anwarul
• Is Part Of: Journal of biomolecular structure & dynamics. Volume 39:Issue 7(2021)
• Page Start: 2595
• Page End: 2606
• Publication Date: 2021-04-09
• Subjects: Nanoporous iron oxide -- nanoparticle -- spectroscopy -- docking -- antibacterial; Biomolecules -- Periodicals; Molecular structure -- Periodicals; Molecular Biology -- Periodicals; Biomechanics -- Periodicals; 572
• DOI: 10.1080/07391102.2020.1751296
• Languages: English
• ISSNs: 0739-1102

Abstract

Abstract: Nanoporous iron oxide (Fe3 O4 ) nanoparticles (NIONPs) have been widely used as promising agents in biomedical applications. Herein, the NIONPs were synthesized by one-step hydrothermal method and well-characterized by FESEM and TEM investigations. Afterwards, their interaction with human serum albumin (HSA) was studied using a wide range of biophysical approaches, including intrinsic and extrinsic fluorescence, far and near UV-CD, and UV-Vis spectroscopic methods as well as molecular docking investigation. Furthermore, the antibacterial effect of NIONPs was examined on the standard strains of the following pathogenic bacteria, Staphylococcus aureus (ATCC 25923), Klebsiella penumoniae (ATCC 33883), Enterococcus faecalis (ATCC 29212) and Pseudomonas aeruginosa (ATCC 27853). The results showed the feasible fabrication of spherical-shaped NIONPs with an average diameter of around 100 nm. Intrinsic fluorescence spectroscopy data depicted that NIONPs formed a complex with HSA by a KSV value of 0.092 (µg/ml) −1 . Extrinsic fluorescence, near UV-CD and UV-vis spectroscopic methods revealed that NIONPs induced some changes on the quaternary structure of HSA, whereas Tm measurement and far UV-CD spectroscopy showed some slight changes on the secondary structure of HSA even in the presence of high concentration of NIONPs. Molecular docking study disclosed that Fe3 O4 nanoclusters with varying morphologies and dimensions could interact with different residues on the surface of HSA molecules. In addition, antibacterial assays exhibited a significant inhibition on both Gram-positive and Gram-negative pathogenic bacteria. In conclusion, these NPs can be used as promising antibacterial agents. Communicated by Ramaswamy H. Sarma