S142 Vastus Lateralis Proteomic Analysis In Muscle Wasted Patients With Copd Using Two-dimensional Fluorescent Electrophoresis. (10th November 2014)

• Record Type: Journal Article
• Authors: Rabinovich, Roberto A; Lahkdar, Ramzi; Drost, Ellen M; Bastos, Ricardo; MacNee, William
• Is Part Of: Thorax. Volume 69(2014)Supplement 2
• Page Start: A75
• Page End: A76
• Publication Date: 2014-11-10
• Subjects: Chest -- Diseases -- Periodicals; Thorax; Chest -- Diseases; Periodicals; Periodicals; 617.54
• DOI: 10.1136/thoraxjnl-2014-206260.148
• Languages: English
• ISSNs: 0040-6376

Abstract

Abstract : Introduction: Muscle wasting, that is present in a subgroup of patients with COPD, is an independent predictor of health related quality of life and survival. The two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) technology is now recognised as an accurate method to determine and quantify proteins. Methods and results: With the aim of identifying proteins potentially involved in the process of muscle wasting, we performed 2D-DIGE protein expression profiling in the vastus lateralis of 10 patients with COPD and low fat free mass index (FFMI) (COPDL ) (FEV1 33 ± 4.3%pred, FFMI 15 ± 0.2 Kg.m -2 ) in comparison with both 8 patients with preserved FFMI (COPDN ) (FEV1 47 ± 7.3%pred, FFMI 19 ± 0.6 Kg.m -2 ) and 9 age and gender-matched healthy sedentary subjects (C) (FEV1 96 ± 4.0%pred, FFMI 20 ± 0.9 Kg.m -2 ). Data analysis was performed using DeCyder software and for protein identification MALDI_TOF mass spectrometry (MS). Ten proteins, whose expression was significantly changed in COPDL, were identified; serum albumin (ALBU), heat shock protein beta-1 (HSPB1), peroxiredoxin-6 (PRDX6), Alpha-crystallin B chain (CRYAB) and Alpha-1-antitrypsin (A1AT) were increased while Histone-lysine N-methyltransferase (DOT1L), Troponin T (TNNT1), Myozenin-1 (MYOZ1), Myosin light chain 1 (MYL1) and mitochondrial ATP synthase subunit alpha (ATPA) were decreased. Conclusion: Our results showed a down-regulation of structural muscle proteins, proteins involved in myofibrillogenesis, cell cycle arrest and energy production and up-regulation of proteins reacting to cell stress and proteins involved in oxidative stress protection. Supported by Chief Scientist Office (CSO) Scotl 06/S1103/5 and FIS PI08/0320 .