Cytotoxic effects of wildfire ashes: In-vitro responses of skin cells. (15th September 2021)

• Record Type: Journal Article
• Authors: Ré, Ana; Rocha, Ana Teresa; Campos, Isabel; Keizer, Jan Jacob; Gonçalves, Fernando J.M.; Oliveira, Helena; Pereira, Joana Luísa; Abrantes, Nelson
• Is Part Of: Environmental pollution. Volume 285(2021)
• Publication Date: 2021-09-15
• Subjects: Wildfire ashes -- Human hazardous potential -- Skin cells (HaCaT) -- Cell viability -- Cell cycle -- In-vitro; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2021.117279
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Wildfires are a complex environmental problem worldwide. The ashes produced during the fire bear metals and PAHs with high toxicity and environmental persistence. These are mobilized into downhill waterbodies, where they can impair water quality and human health. In this context, the present study aimed at assessing the toxicity of mimicked wildfire runoff to human skin cells, providing a first view on the human health hazardous potential of such matrices. Human keratinocytes (HaCaT) were exposed to aqueous extracts of ashes (AEA) prepared from ash deposited in the soil after wildfires burned a pine or a eucalypt forest stand. Cytotoxicity (MTT assay) and changes in cell cycle dynamics (flow cytometry) were assessed. Cell viability decreased with increasing concentrations of AEA, regardless of the ash source, the extracts preparation method (filtered or unfiltered to address the dissolved or the total fractions of contaminants, respectively) or the exposure period (24 and 48 h). The cells growth was also negatively affected by the tested AEA matrices, as evidenced by a deceleration of the progress through the cell cycle, namely from phase G0/G1 to G2. The cytotoxicity of AEA could be related to particulate and dissolved metal content, but the particles themselves may directly affect the cell membrane. Eucalypt ash was apparently more cytotoxic than pine ash due to differential ash metal burden and mobility to the water phase. The deceleration of the cell cycle can be explained by the attempt of cells to repair metal-induced DNA damage, while if this checkpoint and repair pathways are not well coordinated by metal interference, genomic instability may occur. Globally, our results trigger public health concerns since the burnt areas frequently stand in slopes of watershed that serve as recreation sites and sources of drinking water, thus promoting human exposure to wildfire-driven contamination. Graphical abstract: Image 1 Highlights: Wildfire runoffs, reaching hydrographic basins, may pose a human health hazard. The metal and particle burden of runoffs affect human skin cells. Cell viability decreases significantly following exposure to ash extracts. Cell cycle arrests following exposure to ash extracts.