Heteropolysaccharides from S. cerevisiae show anti-adhesive properties against E. coli associated with Crohn's disease. (1st November 2021)

• Record Type: Journal Article
• Authors: Sivignon, Adeline; Yu, Shin-Yi; Ballet, Nathalie; Vandekerckove, Pascal; Barnich, Nicolas; Guerardel, Yann
• Is Part Of: Carbohydrate polymers. Volume 271(2021)
• Publication Date: 2021-11-01
• Subjects: AIEC adherent-invasive Escherichia coli -- CD Crohn's disease -- CEACAM6 carcinoembryonic antigen-related cell adhesion molecule 6 -- YCW yeast cell wall -- Man mannose -- Glc glucose -- PPM phospho-peptido-mannan -- GGM β-glucan/α-glucan/mannan; Mannan-glucan heteropolysaccharides -- Adherent-invasive Escherichia coli -- Saccharomyces cerevisiae -- Anti-adhesive strategy -- NMR analysis; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118415
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The Saccharomyces cerevisiae CNCM I-3856 was previously reported to strongly inhibit adherent-invasive Escherichia coli (AIEC) adhesion to intestinal epithelial cells in vitro and to favor AIEC elimination from the gut in a murine model of Crohn's disease in vivo . In order to identify which cell wall components of yeast are responsible for AIEC elimination, constituent polysaccharides of yeast were isolated and their anti-adhesive ability against AIEC adhesion in vitro was screened. A fraction containing mannan, β-glucan and α-glucan extracted from yeast cell-walls was shown to inhibit 95% of AIEC adhesion in vitro and was thus identified as the strongest anti-adhesive yeast cell wall component. Furthermore, this mannan-glucan-containing fraction was shown to accelerate AIEC decolonization from gut in vivo . This fraction could be proposed as a treatment to eliminate AIEC bacteria in patients with Crohn's disease, a microbial trigger of intestinal inflammation. Highlights: Mannan extracted from yeast inhibits E. coli adhesiveness to intestinal cells in vitro . A soluble fraction containing β-glucan/α-glucan/mannan (GGM) was extracted from S. cerevisiae cell wall. This soluble GGM fraction strongly inhibits E. coli adhesiveness to intestinal cells in vitro . This soluble GGM fraction favors elimination of E. coli from gut in vivo .