Structural elucidation and anti-diabetic osteoporotic activity of an arabinogalactan from Phellodendron chinense Schneid. (1st November 2021)

• Record Type: Journal Article
• Authors: Wang, Nani; Xu, Pingcui; Yao, Weixuan; Zhang, Jiali; Liu, Shufen; Wang, Yongjun; Zhang, Yan
• Is Part Of: Carbohydrate polymers. Volume 271(2021)
• Publication Date: 2021-11-01
• Subjects: AGEs advanced glycation end products -- AUC area under curve -- Ara arabinose -- BMD bone mineral density -- BV/TV bone volume/total volume -- COSY correlated spectroscopy -- DOP diabetic osteoporosis -- FBG fasting blood glucose -- Gal galactose -- GC–MS gas chromatography–mass spectrometry -- Glc glucose -- IC50 half-maximal inhibitory concentration -- HSQC heteronuclear single quantum coherence spectroscopy -- HMBC heteronuclear multiple-bond correlation spectroscopy -- micro-CT micro-computed tomography -- Man mannose -- CML Nε-(carboxymethyl) lysine -- NMR nuclear magnetic resonance -- OGTT oral glucose tolerance test -- PNPG P-nitrophenyl-α-d-glucosidase -- PCP polysaccharides of Phellodendron chinense Schneid. -- PPCP purified polysaccharide of Phellodendron chinense Schneid. -- RAGE receptor for advanced glycation end products -- SEM standard error of mean -- STZ streptozotocin -- Tb.Th trabecular bone thickness -- Tb.N trabecular bone number -- Tb.Sp trabecular bone separation -- UV ultraviolet -- Mw weight-average molecular weight; Phellodendron chinense Schneid. -- Diabetes -- Osteoporosis -- α-Glucosidase -- Blood glucose -- Polysaccharide; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118438
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Phellodendron chinense Schneid. was widely used as a medicinal herb for the treatment of diabetic osteoporosis in China. In this study, an arabinogalactan, named as PPCP-1, was isolated from the bark of Phellodendron chinense Schneid., and purified by DEAE-cellulose DE52 and Sephacryl S-200 HR column chromatography. The structure of PPCP-1 was characterized as a repeating unit consisting of →3)-β-d -Gal p -(1→, →3, 6)-β-d -Gal p -(1→, →5)-α-l -Ara f -(1→, →4)-α-d -Glc p -(1→, →3)-α-d -Glc p -(1→, →4)-α-d -Man p -(1→ with branches of →5)-α-l -Ara f -(1→, →3, 5)-α-l -Ara f -(1→ and terminal α-l -Ara f . Pharmacologically, the oral administration of PPCP-1 preserved osteoporosis associated with hyperglycemia by inhibiting α-glucosidase activity, improving glucose tolerance, decreasing the accumulation of advanced glycation end products (AGEs), as well as down-regulating the expression of receptor for AGEs in tibias of streptozotocin-induced diabetic rats. Collectively, the present study suggested that the arabinogalactan PPCP-1 from Phellodendron chinense Schneid. might potentially be used as functional foods for bone health and/or developed for drug discovery for alleviating diabetic osteoporosis. Highlights: An arabinogalactan PPCP-1 was isolated from Phellodendron chinense Schneid. PPCP-1 was consisted of galactose, glucose, arabinose and mannose. PPCP-1 exerted osteoprotective effects in rats associated with diabetes. PPCP-1 reduced the levels of blood glucose and the expressions of AGEs and RAGE. PPCP-1 was explored to be a candidate inhibitor acting on α-glycosidase.