A chitosan hydrogel sealant with self-contractile characteristic: From rapid and long-term hemorrhage control to wound closure and repair. (1st November 2021)

• Record Type: Journal Article
• Authors: Fang, Wen; Yang, Ling; Hong, Liangjie; Hu, Qiaoling
• Is Part Of: Carbohydrate polymers. Volume 271(2021)
• Publication Date: 2021-11-01
• Subjects: Hemorrhage control -- Wound closure and repair -- Hydrogel -- Self-contraction -- Wet adhesiveness; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118428
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Emergent and long-term hemorrhage control is requisite and beneficial for reducing global mortality and postoperative complications (e.g., second bleeding and adverse tissue adhesion). Despite recent advance in injectable hydrogels for hemostasis, achieving rapid gelation, strong tissue-adhesive property and stable mechanical strength under fluid physiological environment is still challenging. Herein, we developed a novel chitosan hydrogel (CCS@gel) via dynamic Schiff base reaction and mussel-inspired catechol chemistry. The hydrogel possessed high gelation rate (<10 s), strong wet adhesiveness, excellent self-healing performance and biocompatibility. More importantly, the CCS@gel exhibited saline-induced contractile performance and mechanical enhancement, promoting its mechanical property in moist internal conditions. In vivo studies demonstrated its superior hemostatic efficacy for diverse anticoagulated visceral and carotid bleeding scenarios, compared to commercialized fibrin glue. The hydrogel-treated rats survived for 8 weeks with minimal inflammation and postoperative adhesion. These results revealed that the promising CCS@gel would be a facile, efficient and safe sealant for clinical hemorrhage control. Graphical abstract: A novel injectable hydrogel with rapid sol-gel transition, strong wet adhesiveness and cohesiveness is engineered for hemostatic applications as a sealant. The CCS@gel shows unusual volume shrinkage and mechanical enhancement in PBS solutions, and its contraction could induce in vitro wound closure mechanically. Anticoagulated visceral wounds and even bleeding carotid arteries have been successfully sealed by the CCS@gel sealant. Unlabelled Image Highlights: The rapidly-formed hydrogel showed both strong wet adhesiveness and cohesiveness. The sealant performed volume shrinkage and mechanical enhancement in PBS solutions. Anticoagulated visceral wounds and even bleeding carotid arteries were rapidly sealed. Hydrogel-sealed wounds are sustained protected from adverse tissue response. The hydrogel integrates multiple functions, promoting its clinical application.