Allelopathic effects and potential allelochemical of Sargassum fusiforme on red tide microalgae Heterosigma akashiwo. (September 2021)

• Record Type: Journal Article
• Authors: Sun, Siqi; Hu, Shanshan; Zhang, Bo; Sun, Xue; Xu, Nianjun
• Is Part Of: Marine pollution bulletin. Volume 170(2021)
• Publication Date: 2021-09
• Subjects: Sargassum fusiforme -- Heterosigma akashiwo -- Allelopathic effect -- Isolation and purification -- Linolenic acid -- Algicidal mechanism; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2021.112673
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: In this study, we aim to explore the allelochemicals of marine brown algae Sargassum fusiforme and the mechanism of allelopathy effect on Heterosigma akashiwo . Six potential allelopathic substances, palmitic acid, arachidonic acid, α-linolenic acid (ALA), fucosterol, 24-hydroperoxy-24-vinylcholesterol, and saringosterowere, were isolated and identified from S. fusiforme by column chromatography, NMR, TOF-MS and GC‐MS analyses. The ALA and 24-hydroperoxy-24-vinylcholesterol showed remarkable inhibitory activities on H. akashiwo growth. Besides, the allelopathic mechanism between the ALA and H. akashiwo was preliminarily investigated. The results indicated that the activities of SOD and POD of H. akashiwo gradually decreased under high ALA concentration. The reduction of photosynthetic pigments and chlorophyll fluorescence parameters, as well as apparent electron transport rate in H. akashiwo cells, aggravated gradually with increasing the concentration of the ALA. In summary, this study revealed the responses of morphology and physiology of H. akashiwo when exposed by ALA, while revealing the potential of macroalgae in red tide control. Highlights: The effect of Sargassum fusiforme extracts on Heterosigma akashiwo was investigated. Six potential allelochemicals were isolated and identified from S. fusiforme . α-Linolenic acid showed remarkable inhibitory activities on H. akashiwo growth. The allelopathic mechanism of α-linolenic acid on H. akashiwo was studied.