Modified porous carboxymethyl chitin microspheres by an organic solvent-free process for rapid hemostasis. (15th October 2021)

• Record Type: Journal Article
• Authors: Leng, Fan; Chen, Feixiang; Jiang, Xulin
• Is Part Of: Carbohydrate polymers. Volume 270(2021)
• Publication Date: 2021-10-15
• Subjects: Carboxymethyl chitin -- Calcium ions -- Polydopamine -- Aqueous two-phase system -- Hemostatic microsphere; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118348
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Rapid and effective hemorrhage control is essential to reduce mortality following traumatic injuries. Herein we developed an organic solvent-free process to prepare carboxymethyl chitin microsphere (CMCHm) in an aqueous two-phase system through heating and freeze-drying. To further enhance the hemostatic performance of CMCHm, we loaded calcium ions and in-situ polymerized dopamine to get modified hemostatic microspheres CMCHm-Ca 2+ and CMCHm-PDA, respectively. The size of these microspheres was mainly distributed between 50 μm and 150 μm, and the porous microstructure was observed by SEM. The data of in vitro degradation, cell cytotoxicity, and hemolysis test indicated good biocompatibility of these microspheres. Importantly, CMCHm-Ca 2+ and CMCHm-PDA displayed better hemostatic performance compared with CMCHm and the positive controls Yunnan baiyao® and Quickclean®. Especially, the bleeding time was reduced to 59 s (CMCHm-Ca 2+ ) and 45 s (CMCHm-PDA) in the femoral artery/vein cut model, respectively. All these demonstrate CMCHm-Ca 2+ and CMCHm-PDA hold great potential for rapid hemostasis. Graphical abstract: Unlabelled Image Highlights: Carboxymethyl chitin microspheres prepared by an organic solvent-free heating process Two modified microspheres obtained by loading Ca 2+ and dopamine, respectively These microspheres made by a green way have good biocompatibility and degradability. Two modified microspheres showed enhanced hemostatic effect in 3 rat bleeding models These 2 modified porous microspheres hold great potential for clinic rapid hemostasis.