A7.2 Allograft Inflammatory Factor 1 (AIF1) Polymorphisms in French Caucasians with Rheumatoid Arthritis. (25th February 2013)

• Record Type: Journal Article
• Authors: Azzouz, Doua F; Balandraud, Nathalie; Kanaan, Sami B; Auger, Isabelle; Martin, Marielle; Arnoux, Fanny; Roudier, Jean; Lambert, Nathalie C
• Is Part Of: Annals of the rheumatic diseases. Volume 72:Supplement 1(2013)
• Page Start: A48
• Page End: A48
• Publication Date: 2013-02-25
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2013-203221.2
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: Allograft inflammatory factor 1 (AIF1) is a cytoplasmic inflammatory protein encoded within the HLA class III genomic region on chromosome 6 (6p21.3). Although several risk loci for Rheumatoid Arthritis (RA) have been identified by Genome Wide Association Studies (GWAS), none of them involved AIF1 polymorphisms. However, two studies on small cohorts have shown that AIF1 single nucleotide polymorphism (SNP) Rs2269475 (C/T), causing a non-synonymous change of amino acid, is associated with RA (Harney, SM et al, 2008; Pawlik A et al, 2008). Moreover, AIF1 overexpression in inflammatory synovial tissues and macrophages isolated from synovial fluids of patients with RA, confirms its potential role in RA. Objective: We propose to examine the association of the seven most described AIF1 SNPs in our French RA patients. Methods: We have tested 99 Anti-Citrullinated Protein Antibody (ACPA) positive Caucasian RA patients who fulfilled ACR/EULAR criteria and 104 healthy Caucasians. We designed AIF1 primers to specifically amplify the AIF1 gene region containing the 7 SNPs: Rs2844475, Rs4711274, Rs2736182, Rs2736181, Rs2259571, Rs2269475 and Rs13195276. PCR products were sequenced (Cogenics Beckman Coulter) and chromatogram results analysed for the 7 SNPs positions in patients and controls. Patients and controls were genotyped for HLA-DRB1. Results: Two SNPs out of the 7 were associated with RA: Rs4711274 (G/A) and Rs2269475 (C/T). Regarding Rs4711274, G/A and A/A genotypes were increased when compared with controls (p = 0.0005). The minor A allele was strongly associated with RA (p = 0.0005). Regarding Rs22699475, in linkage disequilibrium with the former, we found a similar pattern with increased T/T and C/T genotypes (p = 0.0009) and increased minor T allele frequency (p = 0.0008) in patients with RA. Interestingly, patients carrying the minor associated AIF1 allele expressed HLA-DRB*04 more often than the patient's group carrying the C/C or G/G genotype (63.8% versus 44.4%), although the difference was marginal (p = 0.06). Conclusions: In this study of French Caucasians with RA, we confirmed Rs2269475 association already described in British and Polish Caucasians. Additionally, we find an association with Rs4711274 in linkage disequilibrium with Rs2269475. Intriguingly, such associations have never been found in GWAS.