Investigation into the biological properties, secondary metabolites composition, and toxicity of aerial and root parts of Capparis spinosa L.: An important medicinal food plant. (September 2021)

• Record Type: Journal Article
• Authors: Saleem, Hammad; Khurshid, Umair; Sarfraz, Muhammad; Ahmad, Irshad; Alamri, Abdulwahab; Anwar, Sirajudheen; Alamri, Abdulhakeem S.; Locatelli, Marcello; Tartaglia, Angela; Mahomoodally, Mohamad Fawzi; Zainal Abidin, Syafiq Asnawi; Ahemad, Nafees
• Is Part Of: Food and chemical toxicology. Volume 155(2021)
• Publication Date: 2021-09
• Subjects: Capparis spinose -- Antioxidant -- Secondary metabolites -- Enzyme inhibition -- Bioactive compounds; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2021.112404
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Capparis spinose L. also known as Caper is of great significance as a traditional medicinal food plant. The present work was targeted on the determination of chemical composition, pharmacological properties, and in-vitro toxicity of methanol and dichloromethane (DCM) extracts of different parts of C. spinosa . Chemical composition was established by determining total bioactive contents and via UHPLC-MS secondary metabolites profiling. For determination of biological activities, antioxidant capacity was determined through DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelating assays while enzyme inhibition against cholinesterase, tyrosinase, α-amylase and α-glucosidase were also tested. All the extracts were also tested for toxicity against two breast cell lines. The methanolic extracts were found to contain highest total phenolic and flavonoids which is correlated with their significant radical scavenging, cholinesterase, tyrosinase and glucosidase inhibition potential. Whereas DCM extracts showed significant activity for reducing power, phosphomolybdenum, metal chelation, tyrosinase, and α-amylase inhibition activities. The secondary metabolites profiling of both methanolic extracts exposed the presence of 21 different secondary metabolites belonging to glucosinolate, alkaloid, flavonoid, phenol, triterpene, and alkaloid derivatives. The present results tend to validate folklore uses of C. spinose and indicate this plant to be used as a potent source of designing novel bioactive compounds. Highlights: Chemical, biological and in-vitro toxicological properties of Capparis spinosa extracts were studied. Methanol extracts of both the aerial and root parts were found to have higher total bioactive contents and comparatively higher antioxidant potential. UHPLC-MS and HPLC-PDA analysis revealed the presence of phenolic, alkaloid, glucosinolate, and flavonoid derivatives. The plant was found to present weak to moderate toxicity against the tested cell lines.