Synthesis of Na2S2O4 mediated cleavable affinity tag for labeling of O-GlcNAc modified proteins via azide-alkyne cycloaddition. (15th September 2021)

• Record Type: Journal Article
• Authors: Wang, Jiajia; Dou, Biao; Zheng, Lu; Cao, Wei; Zeng, Xueke; Wen, Yinhang; Ma, Jing; Li, Xia
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 48(2021)
• Publication Date: 2021-09-15
• Subjects: O-GlcNAc -- Proteomics -- Cleavable tag -- Metabolic labeling; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2021.128244
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: A facile and convergent procedure for the synthesis of azobenzene-based probe was reported, which could selectively release interested proteins conducted with sodium dithionite. Besides, the cleavage efficiency is closely associated with the structural features, in which an ortho-hydroxyl substituent is necessary for reactivity. In addition, the azobenzene tag applied in the Ac4 GlcNAz-labled proteins demonstrated high efficiency and selectivity in comparison with Biotin-PEG4 -Alkyne, which provides a useful platform for enrichment of any desired bioorthogonal proteomics.