The n-butanol fraction of the Xiao-Chai-Hu decoction alleviates the endocrine disturbance in the liver of mice exposed to lead. (28th October 2021)

• Record Type: Journal Article
• Authors: Jia, Zheng; Yang, Fan; Liu, Xiaoqing; Zhang, Xiaomeng; Hu, Wanjun; Sheng, Zunlai
• Is Part Of: Journal of ethnopharmacology. Volume 279(2021)
• Publication Date: 2021-10-28
• Subjects: Xiao-Chai-Hu decoction -- Endocrine disorder -- Lead -- Transcriptome -- Liver; XCHD Xiao-Chai-Hu decoction -- XCHD-NBA Xiao-Chai-Hu decoction n-butanol fraction -- XCHD-WR Xiao-Chai-Hu decoction water residue fraction -- EDCs Endocrine-disrupting chemicals -- H.E. Hematoxylin-eosin -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- Tubb2a Tubulin Beta 2A -- Stip stress-induced phosphoprotein 1 -- Cyp4a12a Cytochrome P450 4A12A -- Cyp2c50 Cytochrome P450 2C50 -- Ugt1a1 UDP Glucuronosyltransferase Family 1 Member A1 -- Cyp3a11 Cytochrome P450 3A11 -- Cyp4a12b Cytochrome P450 4A12B -- Ahsa1 Activator of 90 kDa heat shock protein ATPase homolog 1 -- Cyp2c54 Cytochrome P450 2C54 -- Tubb4b Tubulin Beta 4B -- Esr1 estrogen receptor alpha -- Hsp90aa1 Heat Shock Protein 90 Alpha Family Class A Member 1 -- Tuba1a Tubulin alpha 1A -- Tuba1c Tubulin alpha 1C -- Cirbp Cold Inducible RNA Binding Protein -- KDA key driver analysis; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2021.114381
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Lead is a toxic heavy metal that causes health risks globally. However, the mechanism of endocrine poisoning and detoxification of lead poisoning, especially in the liver, still needs to be studied. Xiao-Chai-Hu decoction (XCHD) is regarded as an antidote and an anti-hepatotoxic traditional prescription that has been recorded in the pharmacopeia of the People's Republic of China. Aim of the study: The study aimed to probe the hepatoprotective activity of XCHD in the regulation of endocrine dysfunction in the liver and its molecular mechanism. Materials and methods: The mice from the Institute of Cancer Research (ICR) were exposed to different concentrations of XCHD and lead. Then, serum biochemical indices and liver pathology were analyzed. The key differential genes were detected by qRT-PCR and Western blot. Results: According to the biochemical and histopathological analysis, XCHD-NBA was the most effective in attenuating lead-induced hepatotoxicity. From the transcriptome, we analyzed the key genes of XCHD-NBA in the regulation of lead toxicity, including Tubb2a, Stip1, Cyp4a12a, Cyp2c50, Ugt1a1, Cyp3a11, Cyp4a12b, Ahsa1, Cyp2c54, Tubb4b, Esr1, Hsp90aa1, Tuba1a, Tuba1c, and Hsph1. We also analyzed the main components of XCHD-NBA by LC-MS. Because of their extensive role in regulating the endocrine function, baicalin and glycyrrhizin were identified as the main active components of XCHD in regulating endocrine disorders caused by lead. Conclusions: Lead can disturb the endocrine regulatory process of the liver, while XCHD-NBA alleviates lead-induced liver injury by regulating the endocrine regulatory process. Graphical abstract: Image 1 Highlights: Transcriptome analysis revealed the endocrine pathway which lead disturb. The n-butanol phase of Xiao-Chai-Hu decoction can reduce the endocrine disturbance caused by lead. The main components of n-butanol phase of Xiao-Chai-Hu decoction were identified by LC-MS.