AB0796 Characterization of Mononuclear Cells in Osteoarthritis Synovial Fluid Monocytes. (10th June 2014)

• Record Type: Journal Article
• Authors: Yoo, S.-J.; Kim, J.; Lee, S.; Yoo, I.-S.; Shim, S.-C.; Kwon, M.-H.; Joung, C.-I.; Kang, S.-W.
• Is Part Of: Annals of the rheumatic diseases. Volume 73:Supplement 2(2014)
• Page Start: 1067
• Page End: 1067
• Publication Date: 2014-06-10
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2014-eular.3425
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: In osteoarthritis (OA), synovial inflammation may contribute disease progression. Moreover, monocytes and Toll-like receptors 2 (TLR2) or 4 (TLR4) may play a role in promoting inflammation of OA. However, inflammatory cells in osteoarthritis synovial fluid (OASF) have been rarely studied. Objectives: In order to reveal the inflammatory cells in OASF, we investigated cell markers of mononuclear cells in OA synovial fluid and TLR2 or TLR4 expression in the subtypes of monocyte. Methods: Mononuclear cells were purified by Ficoll density gradient methods from OASFs. Mononuclear cells were stained using anti-CD3, anti-CD4, anti-CD14, anti-CD16, anti-CD64, anti-CD90, anti-TLR4, or anti-TLR2 and analyzed using FACS Canto II. Results: In OASF, there are many CD14+CD16- cells (10.9±10.0%) or CD14+CD16+ cells (21.2±21.8%), CD3+CD4+ cells (16.7±12.9%), CD3+CD4- cells (20.7±12.8%) but rare CD90+ cells (1.7±1.3%). In the majority of CD14+ cells, CD64 and low level CD4 was expressed (CD64, 84.6±1.1%; CD4, 71.0±20.5%). TLR4 expression was higher in CD14+CD16+ cells than in CD14+CD16- cells or CD14-CD16+ cells (mean fluorescence intensity [MFI], CD14+CD16-: 159±128, CD14+CD16+: 307±272, CD14-CD16+: 57±92, p<0.005). TLR2 expression also more increased in CD14+CD16+ cells compared to other subsets (MFI, CD14+CD16-: 117±89, CD14+CD16+: 216±193, CD14-CD16+: -3±34, p<0.005). Conclusions: In OASF, CD3+ T cells and CD14+ monocytes were abundant and majority of CD14+ cells showed CD64 and CD4. Monocyte subtypes are mainly CD14+CD16+ or CD14+CD16- and those subtypes expressed TLR4 and TLR2. Disclosure of Interest: None declared DOI: 10.1136/annrheumdis-2014-eular.3425