AB0016 Association between the hla-b51 antigen and profiles of peripheral t cell subsets in tunisian patients with behcet's disease. (23rd January 2014)

• Record Type: Journal Article
• Authors: Sakly, K.; Lambert, C.; Maatouk, M.; Hammami, S.; Sakly, N.; Harzallah, O.; Mahjoub, S.; Ghedira, I.; Feki, S.
• Is Part Of: Annals of the rheumatic diseases. Volume 72:Supplement 3(2013)
• Page Start: A790
• Page End: A790
• Publication Date: 2014-01-23
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2013-eular.2339
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: Behçet's disease (BD) is a multisystemic inflammatory disorder characterized by spontaneous remissions and relapses [1 ]. An association between Behçet's disease and HLA-B51 has been regarded as the strongest evidence for involvement of genetic factors in its pathogenesis. The exact mechanisms illustrating the involvement of HLA-B51 in BD development are still unknown [2]. Objectives: The aim of this study was to assess possible associations between the expression of HLA-B51 antigen and particular profiles of peripheral T cell subsets in patients with BD. Methods: 30 patients with BD, 19 males and 11 females (mean age: 39.65±10.7 years) were enrolled in this study. Ten of them had active disease. The following monoclonal antibody combination was used to characterize the phenotypes of T cells: anti-CD4-Chrome orange/anti-CD3-PacificBlue/anti-CD62L-PE/anti-CD127-APC-Cy7/anti-CD25-PerCP-Cy5/anti-CD45RA-PCy7 and anti-CD8-APC. Analysis was performed on a FACSDiva™ flow cytometer using FACSDiva™ software. The HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity technique. Results: Our study showed that 38.46 % of patients with BD were HLA-B51 positive (positive group). This group, as compared with HLA-B51 negative patients (negative group), tend to have fewer CD45RA+CD62L+CD4+ naïve T cells (6.77 ± 4.7% versus 8.18 ± 6.5%, p=0.9 ) and higher CD45RA-CD62L+CD4+ central memory T cells (22.7 ± 13.6% versus 13.18 ± 12.2%, p=0.2). The HLA-B51 positive group showed also lesser terminally differentiated CD45RA+CD62L–CD4+ effector memory T cells than the negative group (8.18±3.1% versus 16.86 ± 5%; P = 0.018). However, regarding CD45RA–CD62L–CD4+ effector memory T cells and CD4+CD25brightCD127- T reg cells, no differences were found between the two groups. Moreover, the HLA-B51 positive group seems to be characterized by decreased percentage of CD45RA+CD62L+CD8+ naïve T cells compared to negative group (8.11±3.7% versus 14.02±6.7%; P=0.08). Finally, no differences were found between the two groups regarding CD45RA-CD62L+CD8+ central memory T cells, CD45RA–CD62L–CD8+ effector memory T cells and terminally differentiated CD45RA+CD62L– CD8+ effector memory T cells. Conclusions: Our study showed that HLA-B51 positive patients with BD are characterized by particular profiles of T cells. The understanding of these findings could contribute in elucidating the role of HLA-B51 antigen expression in the pathogenesis of BD. References: Kaneko, F., et al., Behcet's disease (Adamantiades-Behcet's disease). Clin Dev Immunol, 2011. 2011: p. 681956. Disclosure of Interest: None Declared