AB0003 IL-23R and IL-17A Polymorphisms Correlate with Susceptibility of Ankylosing Spondylitis in Chinese Han Population. (15th July 2016) - Yang, B.
Abstract : Background: The association between the IL-23R and IL-17A polymorphisms and ankylosing spondylitis (AS) in a Chinese Han population is still unclear. Objectives: The purpose of this study is to detect the association between IL-23R and IL-17A polymorphisms and AS. Methods: A case–control study consisting of 486 AS patients and 480 healthy controls was performed. We used the high-resolution melting methods (HRM) to genotype the selected five single nucleotide polymorphisms (SNPs), four of them (rs6693831, rs7517847, rs1884444, rs10889677) on the IL-23R gene and one (rs2275913) on the IL-17A gene. Meanwhile the laboratory indexes (RBC, WBC, PLT, MONO%, LYMPH%, NEUT%, ALB, ALP, ALT, AST, BUN, CHOL, CK, CREA, Cys-C, DBIL, GGT, GLB, GLU, HBDH, HDL-C, IBIL, LDH, LDL-C, TBIL, CRP, C3 and C4) were recorded. Results: In this study, patients with genotype CC (p=8.574E-8) and allele C (p=3.206E-31) on SNP rs6693831 (IL-23R) showed decreased risk of AS. The genotype TT (p=4.551E-6) and allele T (p=0.02) on SNP rs1884444 (IL-23R) showed significant lower risk of AS. Individuals carrying the allele A on SNP rs2275913 (IL-17A) showed higher risk of AS [p=0.04, OR (95%CI) = 0.825 (0.690–0.987)]. Significant higher level of CRP was found among AS patients with genotype CT on rs6693831 (χ 2 =7.633, P=0.030). Conclusions: We first demonstrated that rs6693831 and rs1884444 on IL-23R gene and rs2275913 on IL-17A gene are genetic susceptibility factor for AS. We also noticed that patients with CT genotype on rs6693831 have higher CRP level. References: Jiang D, Wubuli A, Hu X, Ikramullah S, Maimaiti A, et al. (2015) The variations of IL-23R are associated with susceptibility and severe clinical forms of pulmonary tuberculosis in Chinese Uygurs. BMC Infect Dis 15: 550. Akbal A, Resorlu H, Gokmen F, Savas Y, Zateri C, et al. (2015) The relationship between C-reactive protein rs3091244 polymorphism and ankylosing spondylitis. Int J Rheum Dis. Almodovar R, Rios V, Ocana S, Gobbo M, Casas ML, et al. (2014) Association of biomarkers of inflammation, cartilage and bone turnover with gender, disease activity, radiological damage and sacroiliitis by magnetic resonance imaging in patients with early spondyloarthritis. Clin Rheumatol 33: 237–241. Wright PB, McEntegart A, McCarey D, McInnes IB, Siebert S, et al. (2015) Ankylosing spondylitis patients display altered dendritic cell and T cell populations that implicate pathogenic roles for the IL-23 cytokine axis and intestinal inflammation. Rheumatology (Oxford). Acknowledgement: We are grateful to the participating AS patients and their families. This research was sponsored by the National Natural Science Foundation of China (Nos. 81301496, and 81202354). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Disclosure of Interest: B. Yang Shareholder of: No, Grant/research support from: No, Consultant for: No, Employee of: No, Paid instructor for: No, Speakers bureau: No, J. Chen: None declared, L. Wang: None declared