Stem cell markers: ABCG2 and MCM2 expression in retinoblastoma. Issue 7 (23rd March 2006)

• Record Type: Journal Article
• Authors: Mohan, A; Kandalam, M; Ramkumar, H L; Gopal, L; Krishnakumar, S
• Is Part Of: British journal of ophthalmology. Volume 90:Issue 7(2006)
• Page Start: 889
• Page End: 893
• Publication Date: 2006-03-23
• Subjects: ABC, ATP binding cassette -- DLP, diode laser photocoagulation -- EBRT, external beam radiation therapy -- LSAB, labelled streptavidin biotin -- MCM, minichromosome maintenance -- PBS, phosphate buffered saline -- TCC, transconjunctival cryopexy; stem cell markers -- retinoblastoma -- chemotherapy -- drug resistance; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.2005.089219
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Backgound/aim: The authors studied the expression of cancer stem cell surface marker, ABCG2, and neural stem cell marker, MCM2, in retinoblastoma and correlated clinicopathologically. Methods: Among 39 retinoblastomas, 18 tumours were not subjected to preoperative/postoperative chemotherapy, 15 tumours underwent postoperative chemotherapy, and six tumours had preoperative chemotherapy. There were 20 tumours with no invasion and 19 tumours with invasion of choroid/optic nerve. ABCG2 and MCM2 expression was studied by immunohistochemistry. Results: ABCG2 was positive in six of six and MCM2 was positive in five of six tumours that had recurred in the orbit or metastasised. ABCG2 was positive in 15/19 tumours with invasion. MCM2 was positive in 16/19 tumours with invasion. Invasive tumours showed higher expression of ABCG2 (p<0.01) and MCM2 (p<0.01) proteins. There was no correlation with differentiation and laterality of the tumours. Non-neoplastic retina was positive for ABCG2 and MCM2. Conclusion: ABCG2 and MCM2 were expressed more in invasive tumours. Further studies are needed to understand the significance of ABCG2 and MCM2 expression in retinoblastoma.