Assessment of a chemiluminescent universal probe for taxonomical and epidemiological investigations of Aeromonas sp isolates. Issue 7 (July 1994)

• Record Type: Journal Article
• Authors: Carey, P E; Eley, A; Wilcox, M H
• Is Part Of: Journal of clinical pathology. Volume 47:Issue 7(1994)
• Page Start: 642
• Page End: 646
• Publication Date: 1994-07
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.47.7.642
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIMS--To assess a chemiluminescent universal probe for taxonomical and epidemiological investigations of Aeromonas sp isolates. METHODS--Total DNA was extracted from 69 well characterised Aeromonas sp strains and digested with the restriction endonucleases Sma I or Pst I. Following electrophoresis, the resulting fragments were transferred to a nylon membrane where they were hybridised to a commercially available universal probe of 16S + 23S rRNA. The banding patterns (ribotypes) were made visible by enhanced chemiluminescence. RESULTS--Both restriction endonucleases produced heterogeneous ribotypes so that no allocation could be made to any of the control genospecies tested. For A hydrophila and A caviae, however, groups of strains (mostly from the same patient) could be identified by indistinguishable banding patterns. A relatively high proportion (36%) of A sobria strains were non-typable. CONCLUSIONS--Although this universal chemiluminescent probe is user friendly, it is unsuitable for taxonomical investigations of Aeromonas sp. It is useful in epidemiological studies of A hydrophila and A caviae, but is of less value for A sobria.