A novel identified Pseudomonas aeruginosa, which exhibited nitrate‐ and nitrite‐dependent methane oxidation abilities, could alleviate the disadvantages caused by nitrate supplementation in rumen fluid fermentation. Issue 4 (11th December 2020)

• Record Type: Journal Article
• Authors: Pang, Jie; Liu, Lihui; Liu, Xiaopeng; Wang, Yi; Chen, Bin; Wu, Shengru; Yao, Junhu; Xu, Xiurong
• Is Part Of: Microbial biotechnology. Volume 14:Issue 4(2021)
• Page Start: 1397
• Page End: 1408
• Publication Date: 2020-12-11
• Subjects: Microbial biotechnology -- Periodicals; Biotechnology; Microbiology; 660.62
• DOI: 10.1111/1751-7915.13726
• Languages: English
• ISSNs: 1751-7915

Abstract

Summary: After the occurrence of nitrate‐dependent anaerobic methane oxidation (AMO) in rumen fluid culture was proved, the organisms that perform the denitrifying anaerobic methane oxidizing (DAMO) process in the rumen of dairy goat were investigated by establishing two enrichment culture systems, which were supplied with methane as the sole carbon source and NaNO3 or NaNO2 as the electron acceptor. Several Operational Taxonomic Units (OTU) belonging to Proteobacteria became dominant in the two enrichment systems. The identified Pseudomonas aeruginosa, which was isolated from the NaNO2 enrichment system, could individually perform a whole denitrifying anaerobic methane oxidizing process. Further in vitro rumen fermentation showed that supplementation with the isolated P. aeruginosa could reduce methane emissions, alleviate the nitrite accumulation and prevent the decrease in propionic acid product caused by nitrate supplementation. Abstract : Proteobacteria became the predominant phylum in the denitrifying anaerobic methane oxidation system of goat rumen fluid. Pseudomonas aeruginosa NWAFUP1 individually perform the whole denitrifying anaerobic methane oxidizing process. P . aeruginosa NWAFUP1 supplementation reduced methane emissions and alleviated the nitrite accumulation and propionic acid synthesis inhibition induced by nitrate supplementation.