MiR‐27b‐5p inhibits BeWo cells fusion by regulating WNT2B and enzyme involved in progesterone synthesis. (10th March 2021)

• Record Type: Journal Article
• Authors: Verma, Sonam; Mishra, Richa; Malik, Ankita; Chaudhary, Piyush; Malhotra, Sudha Saryu; Panda, Amulya K.; Gupta, Satish Kumar
• Is Part Of: American journal of reproductive immunology. Volume 86:Number 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-03-10
• Subjects: forskolin‐mediated BeWo cell fusion -- hCG -- HSD3β1 -- miR‐27b‐5p -- progesterone -- WNT2B; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.13409
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract: Problem: The miRNAs show placenta‐specific expression patterns, which alter during pregnancy‐related complications. In present study, the role of miR‐27b‐5p during forskolin‐mediated BeWo cells fusion has been investigated. Method of Study: The fusion of BeWo cells in response to forskolin treatment (25 µM) was studied by desmoplakin I+II staining. Expression profile of miR‐27b‐5p by qRT‐PCR and its targets HSD3β1 and WNT2B by qRT‐PCR and in Western blot were studied. The effect of overexpression of miR‐27b‐5p and silencing of HSD3β1 & WNT2B by siRNA on forskolin‐mediated BeWo cells fusion and secretion of hCG and progesterone by ELISA was investigated. Results: Time‐dependent down‐regulation in the expression of miR‐27b‐5p in forskolin‐treated BeWo cells has been confirmed by qRT‐PCR. Overexpression of miR‐27b‐5p significantly inhibits forskolin‐mediated BeWo cells fusion as well as hCG & progesterone secretion. HSD3β1 and WNT2B were identified as targets of miR‐27b‐5p and are up‐regulated in forskolin‐treated BeWo cells. Overexpression of miR‐27b‐5p in BeWo cells downregulates their expression. Further, luciferase reporter assay revealed that miR‐27b‐5p directly target expression of both HSD3β1 and WNT2B . Silencing of both HSD3β1 and WNT2B leads to a significant reduction in forskolin‐mediated BeWo cells fusion with concomitant decrease in the secretion of progesterone or/and hCG. Decrease in forskolin‐mediated cells fusion observed in miR‐27b‐5p mimic transfected BeWo cells could be rescued by the overexpression of both HSD3β1 and WNT2B. Conclusion: These observations suggest that reduced miR‐27b‐5p in forskolin‐treated BeWo cells leads to increased secretion of progesterone and hCG due to loss of repressional control on HSD3β1 and WNT2B.