Variable response of arsenic contaminated groundwater microbial community to electron acceptor regime revealed by microcosm based high-throughput sequencing approach. Issue 7 (28th July 2021)

• Record Type: Journal Article
• Authors: Saha, Anumeha; Mohapatra, Balaram; Kazy, Sufia Khannam; Sar, Pinaki
• Is Part Of: Journal of environmental science and health. Volume 56:Issue 7(2021)
• Page Start: 804
• Page End: 817
• Publication Date: 2021-07-28
• Subjects: Microbial diversity -- engal delta plain -- 16S rRNA gene -- taxa recruitment -- functional potential -- As-metabolizing bacteria -- arsenite oxidase; Environmental engineering -- Periodicals; Environmental sciences -- Periodicals; Ecology -- periodicals; Hazardous Substances -- periodicals; 628
• DOI: 10.1080/10934529.2021.1930448
• Languages: English
• ISSNs: 1093-4529

Abstract

Abstract: Arsenic (As) mobilization in alluvial aquifers is facilitated by microbially catalyzed redox transformations that depend on the availability of electron acceptors (EAs). In this study, the response of an As-contaminated groundwater microbial community from West Bengal, India towards varied EAs was elucidated through microcosm based 16S rRNA gene amplicon sequencing. Acinetobacter, Deinococcus, Nocardioides, etc., and several unclassified bacteria ( Ignavibacteria ) and archaea ( Bathyarchaeia, Micrarchaeia ) previously not reported from As-contaminated groundwater of West Bengal, characterized the groundwater community. Distinct shifts in community composition were observed in response to various EAs. Enrichment of operational taxonomic units (OTUs) affiliated to Denitratisoma (NO3 ˉ), Spirochaetaceae (Mn 4+ ), Deinococcus (As 5+ ), Ruminiclostridium (Fe 3+ ), Macellibacteroides (SO4 2 ˉ), Holophagae - Subgroup 7 (HCO3 ˉ), Dechloromonas and Geobacter (EA mixture) was noted. Alternatively, As 3+ amendment as electron donor allowed predominance of Rhizobium . Taxonomy based functional profiling highlighted the role of chemoorganoheterotrophs capable of concurrent reduction of NO3 ˉ, Fe 3+, SO4 2 ˉ, and As biotransformation in As-contaminated groundwater of West Bengal. Our analysis revealed two major aspects of the community, (a) taxa selective toward responding to the EAs, and (b) multifaceted nature of taxa appearing in abundance in response to multiple substrates. Thus, the results emphasized the potential of microbial community members to influence the biogeochemical cycling of As and other dominant anions/cations.