Dansyl–NA3 conjugates for glycoprotein detection through fluorescent tagging and native gel electrophoresis. (7th July 2021)

• Record Type: Journal Article
• Authors: Suchý, Mojmír; Kirby, Alexia; Sabloff, Tara; Mulvihill, Erin E.; Shuhendler, Adam J.
• Is Part Of: New journal of chemistry. Volume 45:Number 29(2021)
• Page Start: 13185
• Page End: 13195
• Publication Date: 2021-07-07
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d1nj02393d
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : An aldehyde-reactive fluorophore has been prepared that can afford the fluorescent detection of serum glycoproteins by native gel electrophoresis. Abstract : A new approach to the fluorescent labelling of glycoproteins was developed utilizing the aldehyde-reactive N -amino anthranilic acid (NA3 ) moiety linked to a dansyl fluorophore. The presented synthetic method is based on the conjugation of isatin as a "masked" anthranilic acid, thus eliminating the need for the use of protecting groups. Fluorescence properties and chemical stability of the prepared molecules were evaluated, resulting in the selection of a conjugate directly connecting the dansyl moiety and aldehyde-reactive NA3 through a sulfonamide linkage for further studies. NaIO4 -mediated oxidation of the glycosylated protein fetuin followed by rapid fluorescent tagging and native gel electrophoresis afforded glycoprotein detection. The detection limit of the described method was 18 ng of protein, providing sensitivity sufficient for the detection of abundant glycosylated serum proteins.