An ultrasensitive fluorescent paper sensor for fast screening of berberine. (7th July 2021) - Guo, Lingling
Abstract : Berberine (BBR) is a highly effective animal feed additive, but it also has adverse side effects on animals and causes potential food safety issues. Based on BBR antibody preparation, fluorescent immunochromatography assay was established for quantitative detection of BBR in feed samples. Abstract : Berberine (BBR) is a highly effective animal feed additive, but it also has adverse side effects on animals and causes potential food safety issues. Here, based on the preparation of a BBR monoclonal antibody, we developed a rapid, simple and sensitive lateral flow immunoassay (LFIA) using gold nanoparticle (GNP)- and fluorescent microsphere (FM)-labelled BBR antibodies for quantitative detection of BBR in feed samples. Under a hand-held optical scanner, the limits of detection of the GNP-LFIA and FM-LFIA were found to be 4.40 μg kg −1 and 2.00 μg kg −1, respectively, in feed samples. The spiked feed samples had acceptable recovery rates using the LFIAs, and the results were further confirmed by liquid chromatography/mass spectrometry. The actual feed sample test results by GNP-LFIA and FM-LFIA also showed good consistency. These two LFIAs provide a good option for fast screening of BBR on-site in feed samples.