Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells. Issue 40 (14th July 2021)

• Record Type: Journal Article
• Authors: Xu, Zhencai; Wang, Xiaofeng; Duan, Tingting; He, Rong; Wang, Fangwu; Zhou, Xuejun
• Is Part Of: RSC advances. Volume 11:Issue 40(2021)
• Page Start: 24669
• Page End: 24672
• Publication Date: 2021-07-14
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra04082k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, we designed a simple coumarin-based fluorescent probe, Cou–HOCl, for detecting HOCl in inflammatory cells. Abstract : Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, a novel coumarin-based fluorescent probe, Cou–HOCl, was prepared for the detection of HOCl. The probe exhibited good selectivity over other analytes, excellent sensitivity with a detection limit of 16 nM, and fast response within 5 s. And further study demonstrated that the probe could be used not only to image exogenous HOCl in various cells, but also to determine the fluctuating levels of HOCl in macrophage cells during inflammation.