Chitinilyticum piscinae sp. nov., isolated from aquaculture water. Issue 7 (26th July 2021)

• Record Type: Journal Article
• Authors: Pei, Tao; Du, Juan; Liu, Yang; Deng, Ming-Rong; Zhu, Honghui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 7(2021)
• Publication Date: 2021-07-26
• Subjects: Chitinilyticum piscinae -- polyphasic taxonomy -- genotypes and phenotypes -- genomic analysis; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004899
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Gram-stain-negative and rod-shaped bacterial strain, designated as 4Y14 T, was isolated from aquaculture water and characterized by using a polyphasic taxonomic approach. Strain 4Y14 T was found to grow at 10–40 °C (optimum, 28 °C), at pH 7.0–9.0 (optimum, 7.0–8.0) and with 0–2 % NaCl (optimum, 1 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 4Y14 T belonged to the genus Chitinilyticum with high levels of similarity to Chitinilyticum litopenaei c1 T (97.8 %) and Chitinilyticum aquatile c14 T (97.2 %). Phylogenomic analysis indicated that strain 4Y14 T formed an independent branch distinct from the two type strains above. Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 4Y14 T and the two type strains were, respectively, 25.3 and 25.0 %, and 81.2 and 80.3 %, which were well below the thresholds of 70 % DDH and 95–96 % ANI for species definition, implying that strain 4Y14 T should represent a novel genospecies. The predominant cellular fatty acids of strain 4Y14 T were summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6 c) and iso-C16 : 0 ; the major polar lipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine; and the sole respiratory quinone was Q-8. The genomic DNA G+C content was 60.1 mol%. Based on the phenotypic and genotypic analyses, strain 4Y14 T is concluded to represent a novel species of the genus Chitinilyticum, for which the name Chitinilyticum piscinae sp. nov. is proposed. The type strain of the species is 4Y14 T (=GDMCC 1.1934 T =KACC 22080 T ).