A new antibacterial nano-system based on hematoporphyrin-carboxymethyl chitosan conjugate for enhanced photostability and photodynamic activity. (1st October 2021)

• Record Type: Journal Article
• Authors: Zhou, Ting; Yin, Yihua; Cai, Weiquan; Wang, Haibo; Fan, Lihong; He, Guanghua; Zhang, Jingli; Jiang, Mengqing; Liu, Jinsheng
• Is Part Of: Carbohydrate polymers. Volume 269(2021)
• Publication Date: 2021-10-01
• Subjects: Carboxymethyl chitosan -- Hematoporphyrin -- Photodynamic action; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118242
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: To promote bactericidal activity, improve photostability and safety, novel antibacterial nanoparticle system based on photodynamic action (PDA) was prepared here through conjugation of photosensitizer hematoporphyrin (HP) onto carboxymethyl chitosan (CMCS) via amide linkage and followed by ultrasonic treatment. The system was stable in PBS (pH 7.4) and could effectively inhibit the photodegradation of conjugated HP because of aggregation-caused quenching effect. ROS produced by the conjugated HP under light exposure could change the structure of nanoparticles by oxidizing the CMCS skeleton and thereby significantly promote the photodynamic activity of HP and its photodynamic activity after 6 h was higher than that of HP·2HCl under the same conditions. Antibacterial experiments showed that CMCS-HP nanoparticles had excellent photodynamic antibacterial activity, and the bacterial inhibition rates after 60 min of light exposure were greater than 97%. Safety evaluation exhibited that the nanoparticles were safe to mammalian cells, showing great potential for antibacterial therapy. Highlights: A hematoporphyrin-carboxymethyl chitosan conjugate (CMCS-HP) was synthesized. An antibacterial nano-system was prepared based on CMCS-HP. The enhanced photostability is attributed to aggregation-caused quenching effect. The photodynamic activity could be enhanced by light-triggered ROS-responsiveness. The system showed photodynamic antibacterial effect and security to NIH-3T3 cells.