Diagnostic characteristics of immature platelet fraction for the assessment of immune thrombocytopenia. Issue 202 (June 2021)

• Record Type: Journal Article
• Authors: Pereira, Karla Nunes; de Carvalho, José Antonio Mainardi; Paniz, Clóvis; Moresco, Rafael Noal; da Silva, José Edson Paz
• Is Part Of: Thrombosis research. Issue 202(2021)
• Page Start: 125
• Page End: 127
• Publication Date: 2021-06
• Subjects: Immature platelet fraction -- Reticulated platelets -- Immune thrombocytopenia -- Diagnostic; Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2021.03.023
• Languages: English
• ISSNs: 0049-3848

Abstract

Abstract: The diagnosis of immune thrombocytopenia (ITP) remains an exclusion, as a specific biomarker is missing. We aimed to investigate the diagnostic characteristics, establish a cut-off point for reticulated platelets, and compare it with the clinical exclusion diagnosis used in the assessment of ITP. Forty-one patients with ITP and 187 healthy individuals were enrolled in Santa Maria, Brazil. Sysmex XE-5000 was used to measure IPF. We obtained an IPF cut-off point of 6.3% with a sensitivity of 92.7% (95% CI: 80.1–98.5) and a specificity of 92.5% (95% CI: 87.8–95.8). The area under the curve was 0.97. The kappa coefficient was 0.85 (95% CI: 0.75–0.95), which shows high agreement between methods. The positive (PPV) and negative predictive values (NPV) were 81.25% and 96.42%, respectively. From the cut-off point, kappa index, PPV, and NPV obtained, it is possible to conclude that IPF can be an efficient laboratory marker for diagnosing ITP.