Microbiome analysis reveals gut microbiota alteration in mice with the effect of matrine. (July 2021)

• Record Type: Journal Article
• Authors: Wu, Haigang; Chen, Qiong; Liu, Jinni; Chen, Xiaoqing; Luo, Houqiang; Ye, Zhaowei; Liu, Jicheng
• Is Part Of: Microbial pathogenesis. Volume 156(2021)
• Publication Date: 2021-07
• Subjects: Gut microbiota -- Matrine -- Amoxicillin -- 16S rRNA -- High-throughput sequencing; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2021.104926
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Mounting evidence revealed the negative effects of abuse of antibiotic including the induction of decreased immunity and dysbacteriosis. Matrine displayed multiple beneficial effects such as anti-inflammatory, antiviral and antibacterial, but studies of its influence on gut microbiota are still insufficient to report. Here, the present study was conducted to investigate the influence of matrine on the gut microbiota of mice and amoxicillin was used as a positive control. A total of 21 cecal samples were obtained from seven groups for high-throughput sequencing analysis based on V3–V4 variable region of 16S rRNA genes. Results revealed that the diversity and abundance of gut microbiota in mice gradually decreased with the increase of the concentration of amoxicillin, whereas matrine administration did not effect the intestinal microbial community structure. Additionally, amoxicillin and matrine supplementation also caused significant changes in the relative abundance of some intestinal bacteria. Specifically, the ratio of Klebsiella and Corynebacterium_1, Bacteroides and Parasutterella in the amoxicillin treated-group were increased as compared to the control group, whereas Muribaculaceae_unclassified, Alistipes and Lactobacillus were significantly decreased. Conversely, matrine administration significantly increased the proportion of beneficial bacteria such as Ruminiclostridium_9, Lachnospiraceae_NK4A136_group and Ruminococcaceae_unclassified . In conclusion, amoxicillin administration could change the microbial community composition and structure by increasing the proportion of pathogenic to beneficial bacteria, whereas matrine could increase the number of beneficial bacteria. Moreover, this study provides a theoretical basis for finding alternatives to antibiotics to decrease bacterial resistance and intestinal flora imbalance. Highlights: Matrine can increased the relative abundance of beneficial bacteria. Matrine may improve the anti-inflammatory properties of the intestine by increasing the number of anti-inflammatory bacteria. Amoxicillin administration not only alter the abundance and diversity of intestinal microbial community but also results in an increase in the proportion of pathogenic and beneficial bacteria.